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BOC Sciences offers an extensive and high-quality range of Boc PNA Monomers and has a quality control system in place to analyze Boc PNA Monomers by MS, NMR, and HPLC to validate their structure and purity. We work with our customers to understand their needs and provide customized Boc PNA monomer synthesis services. PNA of different lengths, sequences and modifications are also available by using our Boc PNA monomers according to customer requirements.
Fig.1 Synthesis of Boc-protected PNA monomers. (Amant et al., 2012)
Boc PNA monomer is a protected chemical monomer used in the construction of peptide nucleic acid (PNA), an artificial nucleic acid molecule with high specificity and affinity, whose backbone consists of N-(2-aminoethyl)formamide monomers. Boc (tert-butyloxycarbonyl) is a commonly used protecting group for the protection of amino groups in chemical reactions.
The introduction of Boc PNA monomers has made PNA synthesis more flexible and versatile. By introducing Boc protecting groups at specific positions in the PNA sequence, the synthesis and modification process of PNA can be controlled to obtain the desired sequence and function. In addition, the introduction of Boc protecting groups can increase the solubility and stability of PNA and improve its performance in biological applications.
Boc PNA monomers are mainly classified according to the base composition and are mainly divided into the following categories.
A (Adenine) monomers contain adenine bases and are used to form base pairs with thymine (T) or uracil (U). For example, Boc-PNA-A(Z)-OH.
T (thymine)-like monomers contain thymine bases and are used to form base pairs with adenine (A). For example, Boc-PNA-T-OH.
G (guanine) monomers contain guanine bases and are used to form base pairs with cytosine (C). For example, Boc-PNA-G(Z)-OH.
C (cytosine) monomers contain cytosine bases and are used to form base pairs with guanine (G). For example, Boc-PNA-C(Z)-OH.
Boc in Boc-PNA monomers is mainly used to protect the amino (-NH2) functional group, which is protected for several reasons.
Amino groups can easily react non-specifically with other reactants in chemical synthesis, leading to the occurrence of side reactions. By introducing a protecting group, the amino group can be prevented from participating in non-specific reactions, thus protecting the integrity of the target compound.
The introduction of a protecting group can make a specific site in the synthesis process selective for a particular reaction. This is important for the protection and deprotection of specific functional groups during multi-step synthesis to ensure that the desired chemical transformation takes place at a specific site.
Boc PNA monomer is highly chemically stable and resistant to enzymatic degradation and chemical changes in the environment. This gives Boc-PNA long-lasting biological activity in in vitro and in vivo applications.
Boc PNA monomer shows high affinity and specific binding capacity due to PNA's base pairing ability similar to DNA and RNA.
Since Boc PNA monomer is uncharged, it can more easily cross cell membranes and tissue barriers relative to charged DNA or RNA, achieving higher cell permeability.
Boc PNA monomer shows high selectivity in hybridization with DNA and RNA, being able to distinguish even single base differences.
Due to its unnatural chemical structure, Boc PNA monomer shows high resistance to many nucleases, which increases stability and activity in organisms.
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