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Fmoc PNA Monomers

Fmoc-PNA-A(Boc)-OH

CAS 511534-99-9
Catalog BAT-014340
Molecular Weight 615.65
Molecular Formula C31H33N7O7
Fmoc-PNA-A(Boc)-OH

Fmoc-PNA-A(Bhoc)-OH

CAS 186046-82-2
Catalog BAT-008536
Molecular Weight 725.7
Molecular Formula C40H35N7O7
Fmoc-PNA-A(Bhoc)-OH

Fmoc-PNA-C(Boc)-OH

CAS 172405-61-7
Catalog BAT-014341
Molecular Weight 591.62
Molecular Formula C30H33N5O8
Fmoc-PNA-C(Boc)-OH

Fmoc-PNA-C(Bhoc)-OH

CAS 186046-81-1
Catalog BAT-008537
Molecular Weight 701.7
Molecular Formula C39H35N5O8
Fmoc-PNA-C(Bhoc)-OH

Fmoc-PNA-G(Boc)-OH

CAS 1052677-90-3
Catalog BAT-014342
Molecular Weight 631.65
Molecular Formula C31H33N7O8
Fmoc-PNA-G(Boc)-OH

Fmoc-PNA-G(Bhoc)-OH

CAS 186046-83-3
Catalog BAT-014015
Molecular Weight 741.76
Molecular Formula C40H35N7O8
Fmoc-PNA-G(Bhoc)-OH

Fmoc-PNA-T-OH

CAS 169396-92-3
Catalog BAT-008538
Molecular Weight 506.5
Molecular Formula C26H26N4O7
Fmoc-PNA-T-OH

Fmoc-PNA-U-OH

CAS 959151-70-3
Catalog BAT-014343
Molecular Weight 492.49
Molecular Formula C25H24N4O7
Fmoc-PNA-U-OH

Fmoc-PNA-J(Boc)-OH

CAS 1095275-70-9
Catalog BAT-014347
Molecular Weight 591.62
Molecular Formula C30H33N5O8
Fmoc-PNA-J(Boc)-OH

Fmoc-PNA-J(Bhoc)-OH

Catalog BAT-014346
Molecular Weight 701.74
Molecular Formula C39H35N5O8
Fmoc-PNA-J(Bhoc)-OH

Fmoc-PNA-M(Boc)-OH

CAS 1417611-27-8
Catalog BAT-014345
Molecular Weight 574.63
Molecular Formula C31H34N4O7
Fmoc-PNA-M(Boc)-OH

Fmoc-PNA-M(Bhoc)-OH

Catalog BAT-014344
Molecular Weight 684.74
Molecular Formula C40H36N4O7
Fmoc-PNA-M(Bhoc)-OH

Fmoc-PNA-D(tetraBoc)-OH

CAS 49564-57-0
Catalog BAT-014349
Molecular Weight 230.29
Molecular Formula C46H58N8O13
Fmoc-PNA-D(tetraBoc)-OH

Fmoc-PNA-D(tetraBhoc)-OH

Catalog BAT-014348
Molecular Weight 1371.47
Molecular Formula C82H66N8O13
Fmoc-PNA-D(tetraBhoc)-OH

Fmoc-PNA-E-OH

Catalog BAT-014350
Molecular Weight 476.49
Molecular Formula C25H24N4O6
Fmoc-PNA-E-OH

Fmoc-PNA-P-OH

Catalog BAT-014351
Molecular Weight 505.53
Molecular Formula C26H27N5O6
Fmoc-PNA-P-OH

Fmoc-PNA-Maleimide-OH

Catalog BAT-014373
Molecular Weight 491.49
Molecular Formula C26H25N3O7
Fmoc-PNA-Maleimide-OH

Guanidino-G-Clamp-PNA

Catalog BAT-014374
Molecular Weight 950.96
Molecular Formula C50H46N8O12
Guanidino-G-Clamp-PNA

9-Fluorenylmethyl chloroformate (Fmoc) PNA monomers refer to the amino acids used to synthesize PNA by using the Fmoc peptide synthesis technology.

Introduction

PNA is a kind of DNA analog that can recognize and combine DNA or RNA sequences to form a stable double helix structure.

Fmoc is used as the protecting group of α-amino of amino acid. Its advantage is that it is stable under acidic conditions which is not affected by trifluoroacetic acid (TFA) and other reagents, and can be deprotected by mild alkali treatment. At the same time, the Fmoc method has fewer side reactions, high yield, and the Fmoc group itself has characteristic UV absorption, which is easy to monitor and control the reaction.

Synthesis Steps

The amino backbone of Fmoc PNA monomers is protected by Fmoc, which enables PNA to bind to peptides and markers such as biotin or fluorescent dyes. Each synthesis cycle takes about 30 minutes, mainly including the following steps:

  1. Install the column. Unlike the conventional peptide synthesis column that connects the synthesized C-terminal amino acid to the solid phase support, PNA synthesis column only contains polystyrene solid phase support.
  2. Washing. Washing PNA synthetic column with dimethylformamide (DMF)
  3. De close. Removal of Fmoc protective group with DMF
  4. Washing. Remove excess reagent by washing with DMF.
  5. Activation and coupling. In the presence of a mixture of diisopropylethylamine and hexafluorophosphate, the newly synthesized PNA monomer is activated and coupled to the solid phase resin.
  6. Washing. Wash with DMF to remove excess reagent.
  7. Cap. The unreacted gene is capped by acetic anhydride dissolved in DMF.
  8. Washing. Wash with DMF to remove excess reagent.
  9. The PNA is incubated with trifluoric acid and m-cresol mixture for cutting and final deprotection.

Application

Fmoc compound is selected to protect PNA monomer. After one monomer reacts at each point, its synthesis efficiency is tested by bromophenol blue color reaction. The efficiency of the synthesized PNA oligomer can reach 95%.

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