9-Fluorenylmethyl chloroformate (Fmoc) PNA monomers refer to the amino acids used to synthesize PNA by using the Fmoc peptide synthesis technology.
PNA is a kind of DNA analog that can recognize and combine DNA or RNA sequences to form a stable double helix structure.
Fmoc is used as the protecting group of α-amino of amino acid. Its advantage is that it is stable under acidic conditions which is not affected by trifluoroacetic acid (TFA) and other reagents, and can be deprotected by mild alkali treatment. At the same time, the Fmoc method has fewer side reactions, high yield, and the Fmoc group itself has characteristic UV absorption, which is easy to monitor and control the reaction.
The amino backbone of Fmoc PNA monomers is protected by Fmoc, which enables PNA to bind to peptides and markers such as biotin or fluorescent dyes. Each synthesis cycle takes about 30 minutes, mainly including the following steps:
Fmoc compound is selected to protect PNA monomer. After one monomer reacts at each point, its synthesis efficiency is tested by bromophenol blue color reaction. The efficiency of the synthesized PNA oligomer can reach 95%.