1. A general method for the synthesis of 5'-monophosphates of DNA fragments via phosphotriester intermediates
G A van der Marel, C A van Boeckel, G Wille, J H van Boom Nucleic Acids Res. 1982 Apr 10;10(7):2337-51. doi: 10.1093/nar/10.7.2337.
A new and attractive phosphorylation procedure which allows the introduction, via phosphotriester intermediates, of 5'-phosphate functions of DNA fragments is described. The method is based on the activation of bifunctional phosphorylating agents with 1-hydroxybenzotriazole. The approach will be exemplified by the synthesis of pACGC using four different 5'-phosphotriester intermediates.
2. Chemical synthesis of r(GCAUGC) for high-resolution 1H-NMR studies
E Happ, C Scalfi-Happ, G M Clore, A M Gronenborn Nucleic Acids Symp Ser. 1987;(18):265-8.
The chemical synthesis of a short RNA duplex, to be used for 500 MHz 1H-NMR experiments, is presented. The target oligonucleotide was constructed in a blockwise fashion according to the benzotriazolyl phosphotriester approach in solution. The CPTr group, 4,4',4"-tris(4,5-dichlorophthalimido)trityl, was used for temporary protection of the 5'-OH; this blocking group was removed by buffered hydrazine without harming any other protecting group.
3. Synthesis of short RNA fragments by the benzotriazolyl phosphotriester approach
C T Wreesmann, A Fidder, G A van der Marel, J H van Boom Nucleic Acids Res. 1983 Dec 10;11(23):8389-405. doi: 10.1093/nar/11.23.8389.
Analytical and experimental evidence will be presented to show that addition of a slight excess of 2-chlorophenyl-0,0-bis[1-benzotriazolyl]phosphate to a properly 2',5'-protected ribonucleoside gives an intermediate 3'-phosphotriester, which, after addition of a 2'-protected ribonucleoside, affords a dimer containing solely a 3'-5'-internucleotide linkage. The above phosphorylation procedure has been used in the synthesis of the pentamer UpApCpGpC.
