1.Evaluation of photodynamic therapy using topical aminolevulinic acid hydrochloride in the treatment of condylomata acuminate.
Zhang Z1, Lu XN1, Liang J1, Tang H1, Yang YS1, Zhu XH1, Du J1, Shen YY1, Xu JH1. Int J Clin Exp Med. 2015 Apr 15;8(4):6517-21. eCollection 2015.
OBJECTIVES: To investigate the efficacy and safety of topical application of 5-aminolaevulinic acid (ALA) photodynamic therapy (PDT) for the treatment of condylomata acuminata (CA) in larger population.
2.Fluorescent detection of peritoneal metastasis in human colorectal cancer using 5-aminolevulinic acid.
Kondo Y1, Murayama Y1, Konishi H1, Morimura R1, Komatsu S1, Shiozaki A1, Kuriu Y1, Ikoma H1, Kubota T1, Nakanishi M1, Ichikawa D1, Fujiwara H1, Okamoto K1, Sakakura C1, Takahashi K2, Inoue K2, Nakajima M2, Otsuji E1. Int J Oncol. 2014 Jul;45(1):41-6. doi: 10.3892/ijo.2014.2417. Epub 2014 May 6.
A precise diagnosis of peritoneal dissemination is necessary to determine the appropriate treatment strategy for colorectal cancer. However, small peritoneal dissemination is difficult to diagnose. 5-aminolevulinic acid (5-ALA) is an intermediate substrate of heme metabolism. The administration of 5-ALA to cancer patients results in tumor-specific accumulation of protoporphyrin IX (PpIX), which emits red fluorescence with blue light irradiation. We evaluated the usefulness of photodynamic diagnosis (PDD) using 5-ALA to detect the peritoneal dissemination of colorectal cancer. EGFP-tagged HT-29 cells were injected into the peritoneal cavity of BALB/c nude mice. After 2 weeks, the mice were given 5-ALA hydrochloride, and metastatic nodules in the omentum were observed with white light and fluorescence images. Twelve colorectal cancer patients suspected to have serosal invasion according to preoperative computed tomography (CT) were enrolled in this study.
3.Intraoperative Detection of Superficial Liver Tumors by Fluorescence Imaging Using Indocyanine Green and 5-aminolevulinic Acid.
Kaibori M1, Matsui K2, Ishizaki M2, Iida H2, Okumura T2, Sakaguchi T2, Inoue K2, Ikeura T3, Asano H4, Kon M2. Anticancer Res. 2016 Apr;36(4):1841-9.
BACKGROUND/AIM: Indocyanine green (ICG) and the porphyrin precursor 5-aminolevulinic acid (5-ALA) have been approved as fluorescence imaging agents in the clinical setting. This study evaluated the usefulness of fluorescence imaging with both ICG and 5-ALA for intraoperative identification of latent small liver tumors.
4.Type 2 diabetic conditions in Otsuka Long-Evans Tokushima Fatty rats are ameliorated by 5-aminolevulinic acid.
Sato T1, Yasuzawa T2, Uesaka A2, Izumi Y3, Kamiya A4, Tsuchiya K4, Kobayashi Y2, Kuwahata M2, Kido Y5. Nutr Res. 2014 Jun;34(6):544-51. doi: 10.1016/j.nutres.2014.04.013. Epub 2014 Apr 26.
A precursor of protoporphyrin IX, 5-aminolevulinic acid (5-ALA) is used as a prodrug for photodiagnosis and photodynamic therapy. Recently, it has been shown that 5-ALA reduces glucose levels during fasting and after glucose loading in prediabetic subjects. We hypothesized that 5-ALA ameliorates diabetic conditions through mitochondrial changes in visceral adipose tissue. In order to explore the metabolic effects on the type 2 diabetic state, we administered ALA hydrochloride in combination with sodium ferrous citrate to Otsuka Long-Evans Tokushima Fatty (OLETF) rats at intragastric doses of 20 and 300 mg kg(-1) d(-1) for 6 weeks. The administration of 300 mg kg(-1) d(-1) of 5-ALA improved glucose intolerance, hypertriglyceridemia, and hyperleptinemia in OLETF rats more effectively than the administration of an equivalent dose of metformin, in accordance with reductions in food intake and body weight. Furthermore, the weight of the retroperitoneal fat tended to decrease and cellular mitochondrial content of the fat was markedly reduced by the 5-ALA administration, showing a positive correlation.