1. Cloning and analysis of a transcript derived from two contiguous genes of the cathelicidin family
M Scocchi, S Wang, R Gennaro, M Zanetti Biochim Biophys Acta. 1998 Jul 9;1398(3):393-6. doi: 10.1016/s0167-4781(98)00076-1.
Bac4 is a bovine cathelicidin gene contiguous to another member of this family named Bac7. Although mutations in the sequence suggested that Bac4 gene might be non-functional, primers based on Bac4 specific sequences allowed amplification of a 900 bp cDNA. The transcript comprises the sequences of exons 1, 2 and 3 of Bac7, and of exons 2, 3 and 4 of Bac4 gene and may result from a weak termination control of the transcription of the upstream Bac7 gene.
2. Calcium entry-dependent oscillations of cytoplasmic calcium concentration in cultured endothelial cell monolayers
R E Laskey, D J Adams, M Cannell, C van Breemen Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1690-4. doi: 10.1073/pnas.89.5.1690.
Bovine endothelial cell monolayers grown to confluence and stimulated with bradykinin responded with periodic fluctuations in intracellular Ca2+ concentration ([Ca2+]i) when exposed to K(+)-free Hepes-buffered saline. The fluctuations in [Ca2+]i measured with fura-2 were synchronized among the population of cells observed and were sensitive to extracellular Ca2+ concentration ([Ca2+]o). Thapsigargin, which inhibits the endoplasmic reticular Ca2(+)-ATPase, did not inhibit the [Ca2+]i oscillations. Removal of extracellular Ca2+ or inhibition of Ca2+ entry by using La3+ or 1-(beta- [3-(4-methoxyphenyl)proproxy]-4-methoxyphenethyl)-1H-imidazole hydrochloride (SKF 96365) abolished the [Ca2+]i oscillations in endothelial cell monolayers. The fluctuations in [Ca2+]i were therefore dependent on Ca2+ influx rather than Ca2+ mobilization from intracellular stores. Simultaneous measurements of membrane potential (Em) using the potential-sensitive bisoxonol dye bis(1,3-dibutylbarbituric acid)trimethine oxonol [Di-BAC4(3)] and [Ca2+]i using fura-2 showed that Em oscillated at the same frequency as the fluctuations in [Ca2+]i. The peak depolarization signal coincided with the maximum rate of increase in the [Ca2+]i signal. Oscillations in the Em signal were inhibited by removal of Ca2+ or by addition of 1 mM Ni2+ to the external solution. Taken together, these observations suggest that the change in Em is the consequence of oscillatory changes in a membrane conductance that also allows Ca2+ to enter the cell. Oscillations in the DiBAC4(3) signal may reflect a rhythmic entry of Ca2+ through nonselective cation channels.
