Boc-Asp-OMe
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Boc-Asp-OMe

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Category
BOC-Amino Acids
Catalog number
BAT-007632
CAS number
98045-03-5
Molecular Formula
C10H17NO6
Molecular Weight
247.20
Boc-Asp-OMe
IUPAC Name
(3S)-4-methoxy-3-[(2-methylpropan-2-yl)oxycarbonylamino]-4-oxobutanoic acid
Synonyms
Boc-L-aspartic acid α-methyl ester; (3S)-3-{[(tert-butoxy)carbonyl]amino}-4-methoxy-4-oxobutanoic acid; N-Boc-L-aspartic Acid 1-Methyl Ester; 1-Methyl N-Boc-L-Aspartate; N-tert-Butoxycarbonylaspartic acid a-methyl ester; N-BOC ASP(OH)OME
Appearance
White powder
Purity
≥ 99% (HPLC)
Density
1.209 g/cm3 (Predicted)
Melting Point
86-93 °C
Boiling Point
407.1±40.0 °C (Predicted)
Storage
Store at 2-8 °C
InChI
InChI=1S/C10H17NO6/c1-10(2,3)17-9(15)11-6(5-7(12)13)8(14)16-4/h6H,5H2,1-4H3,(H,11,15)(H,12,13)/t6-/m0/s1
InChI Key
IWFIVTBTZUCTQH-LURJTMIESA-N
Canonical SMILES
CC(C)(C)OC(=O)NC(CC(=O)O)C(=O)OC

Boc-Asp-OMe, a derivative of the amino acid aspartic acid, plays a significant role in peptide synthesis and biotechnological research. Here are four key applications of Boc-Asp-OMe, presented with high perplexity and burstiness:

Peptide Synthesis: Serving as a cornerstone in peptide synthesis, Boc-Asp-OMe is a pivotal building block essential for creating complex peptides. The Boc protective group shields the amino acid, maintaining its stability and inertness during coupling reactions. This methodical approach facilitates the gradual assembly of intricate peptides, crucial for advancing studies in biochemistry and drug discovery.

Protein Engineering: Embracing the realm of protein engineering, Boc-Asp-OMe is harnessed to introduce aspartic acid residues into engineered proteins. The esterified carboxyl group allows for targeted deprotection and integration at specific loci, enabling researchers to craft proteins with tailored functionalities. This process enhances our comprehension of protein structure-function relationships, shedding light on the intricate dynamics within biological systems.

Pharmaceutical Development: Vital in the realm of pharmaceutical development, Boc-Asp-OMe plays a pivotal role in crafting therapeutic peptides and peptide-based drugs. Its versatile nature permits precise modifications to peptide sequences, enhancing pharmacokinetic properties and target specificity. This capability is paramount in developing novel treatments for various ailments, including cancer and infectious diseases.

Biotechnological Assays: In the domain of biotechnological assays, Boc-Asp-OMe serves as a valuable tool for studying enzyme activities and exploring protein interactions. Leveraging the protected aspartic acid derivative in substrate design for enzymatic reaction profiling, researchers gain insights into enzyme mechanisms and conduct screenings for enzyme inhibitors in drug discovery endeavors. These assays play a crucial role in unraveling the intricacies of biochemical processes, fueling advancements in biotechnological research.

1. Caspase-independent cell death induced by anti-CD2 or staurosporine in activated human peripheral T lymphocytes
O Déas, C Dumont, M MacFarlane, M Rouleau, C Hebib, F Harper, F Hirsch, B Charpentier, G M Cohen, A Senik J Immunol. 1998 Oct 1;161(7):3375-83.
We examined the effects of the cell-permeable, broad spectrum peptide caspase inhibitors, benzyloxycarbonyl-Val-Ala-Asp(OMe)-fluoromethyl ketone (Z-VAD.fmk), and BOC-Asp(OMe)-fluoromethyl ketone (BOC-D.fmk), on apoptosis induced by anti-CD2, anti-Fas, and the protein kinase inhibitor staurosporine in activated human peripheral T lymphocytes. We monitored ultrastructural, flow cytometric, and biochemical apoptotic changes, including externalization of phosphatidylserine, cleavage of poly(ADP-ribose) polymerase (PARP) and lamins, activation of caspase-3 and caspase-7, decrease in mitochondrial membrane potential, and DNA fragmentation. Z-VAD.fmk and BOC-D.fmk completely inhibited all the biochemical and ultrastructural changes of apoptosis in anti-Fas-treated cells. In marked contrast, neither Z-VAD.fmk nor BOC-D.fmk inhibited CD2- or staurosporine-mediated cell shrinkage, dilatation of the endoplasmic reticulum (seen in anti-CD2-treated cells), externalization of phosphatidylserine, and loss of mitochondrial membrane potential that accompanied cell death. However, these inhibitors did inhibit the cleavage of PARP and lamins and the formation of hypodiploid cells, and partially inhibited chromatin condensation. These results demonstrate that in activated T cells, anti-CD2 and staurosporine induce a caspase-independent cell death pathway that exhibits prominent cytoplasmic features of apoptosis. However, caspase activation is required for the proteolytic degradation of nuclear substrates such as PARP and lamins together with the DNA fragmentation and extreme chromatin condensation that occur in apoptotic cells.
2. (-)-Anonaine induces apoptosis through Bax- and caspase-dependent pathways in human cervical cancer (HeLa) cells
Chung-Yi Chen, Tsan-Zon Liu, Wei-Chang Tseng, Fung-Jou Lu, Ray-Ping Hung, Chi-Hung Chen, Ching-Hsein Chen Food Chem Toxicol. 2008 Aug;46(8):2694-702. doi: 10.1016/j.fct.2008.04.024. Epub 2008 Apr 27.
(-)-Anonaine has been shown to have some anticancer activities, but the mechanisms of (-)-anonaine inducing cell death of human cancer cells is not fully understood. We investigated the mechanisms of apoptosis induced by (-)-anonaine in human HeLa cancer cells. Treatment with (-)-anonaine induces dose-dependent DNA damage that is correlated with increased intracellular nitric oxide, reactive oxygen species, glutathione depletion, disruptive mitochondrial transmembrane potential, activation of caspase 3, 7, 8, and 9, and poly ADP ribose polymerase cleavage. Our data indicate that (-)-anonaine up-regulated the expression of Bax and p53 proteins in HeLa cancer cells. The apoptosis and expression of Bax induced by (-)-anonaine could be inhibited when the HeLa cells were pretreated with Boc-Asp(OMe)-fmk, which is a broad caspases inhibitor. There was no obvious DNA damage in the (-)-anonaine-treated Madin-Darby canine kidney and Vero cell lines. Both Madin-Darby canine kidney and Vero cell lines are kidney epithelial cellular morphology. These results suggest that (-)-anonaine might be considered a potent compound for chemotherapy against cervical cancer or a health food supplement for cancer chemoprevention.
3. Crystal structure of N-{ N-[ N-( tert-but-oxy-carbon-yl)-l-α-aspart-yl]-l-α-aspart-yl}-l-α-aspartic acid 14,24,34-trimethyl ester 31-2-oxo-2-phenyl-ethyl ester {Boc-[Asp(OMe)]3-OPac}
Takuma Kato, Saki Kishimoto, Akiko Asano, Mitsunobu Doi Acta Crystallogr E Crystallogr Commun. 2019 Apr 9;75(Pt 5):585-588. doi: 10.1107/S2056989019004596. eCollection 2019 May 1.
In the title homotripeptide {Boc-[Asp(OMe)]3-OPac}, C28H37N3O13, all peptide bonds adopt an s-trans conformation with respect to the N-H and C=O groups. In the crystal, N-H⋯O hydrogen bonds result in an infinite parallel β-sheet structure running along the b-axis direction. The Boc protecting group at the N-terminus of the peptide is disordered over two sites with occupancy factors of 0.504 (5) and 0.496 (5).
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