1.Ruminal Infusions of Cobalt EDTA Modify Milk Fatty Acid Composition via Decreases in Fatty Acid Desaturation and Altered Gene Expression in the Mammary Gland of Lactating Cows.
Leskinen H1, Viitala S1, Mutikainen M1, Kairenius P1, Tapio I1, Taponen J2, Bernard L3, Vilkki J1, Shingfield KJ4. J Nutr. 2016 Apr 13. pii: jn226100. [Epub ahead of print]
BACKGROUND: Intravenous or ruminal infusion of lithium salt of cobalt EDTA (Co-EDTA) or cobalt-acetate alters milk fat composition in cattle, but the mechanisms involved are not known.
2.Separation of dynorphin peptides by capillary electrochromatography using a polydiallyldimethylammonium chloride gold nanoparticle-modified capillary.
Al-Hossaini AM1,2, Suntornsuk L2,3, Lunte SM1,4,2. Electrophoresis. 2016 Apr 30. doi: 10.1002/elps.201600006. [Epub ahead of print]
Dynorphin A (Dyn A) is an endogenous opioid peptide found in blood and CNS tissue at very low concentrations. Elevated levels of Dyn A due to different disease states, for example, neurodegenerative disease, have been linked to toxic nonopioid activity. Capillary electrophoresis (CE) is a powerful technique that can achieve high efficiency separations of charged analytes. However, CE has limited use for the analysis of basic proteins and peptides, due to their adsorption onto the inner surface of the fused silica at pHs below their pI. This adsorption can lead to a loss of efficiency, irreproducibility of migration times, and peak tailing. To obviate this problem, a polydiallyldimethylammonium chloride-stabilized gold nanoparticle-coated capillary was investigated for the separation of dynorphin metabolites. The positively charged gold nanoparticles (GNP) minimized unwanted adsorption of the positively charged peptides onto the surface of the fused silica capillary.
