1.Controlled Inactivation of Trichophyton rubrum Using Shaped Electrical Pulse Bursts: Parametric Analysis.
Novickij V1, Grainys A1, Švedienė J2, Paškevičius A2,3, Novickij J1. Biotechnol Prog. 2016 Apr 12. doi: 10.1002/btpr.2276. [Epub ahead of print]
The dermatophytes infect the skin by adherence to the epidermis followed by germination, growth and penetration of the fungal hyphae within the cells. The aim of this study was to investigate the efficacy of the pulsed electric fields (PEF) of controlled inactivation of Trichophyton rubrum (ATCC 28188). In this work we have used bursts of the square wave PEF pulses of different intensity (10-30 kV/cm) to induce the irreversible inactivation in vitro. The electric field pulses of 50 µs and 100 µs have been generated in bursts of 5, 10 and 20 pulses with repetition frequency of 1 Hz. The dynamics of the inactivation using different treatment parameters were studied and the inactivation map for the T. rubrum has been defined. Further, the combined effect of pulsed electric fields with the antifungal agents itraconazole, terbinafine and naftifine HCl was investigated. It has been demonstrated that the combined effect results in the full inactivation of T.
2.Mucosal acidification increases hydrogen sulfide release through up-regulating gene and protein expressions of cystathionine gamma-lyase in the rat gastric mucosa.
Mard SA1, Veisi A2, Ahangarpour A2, Gharib-Naseri MK2. Iran J Basic Med Sci. 2016 Feb;19(2):172-7.
OBJECTIVES: This study was performed to investigate the effects of mucosal acidification on mRNA expression and protein synthesis of cystathionine gamma lyase (CSE), cystathionine beta synthase (CBS), and mucosal release of H2S in gastric mucosa in rats.
3.Design and Evaluation of Proniosomes As A Carrier for Ocular Delivery of Lomefloxacin HCl.
Khalil RM1, Abdelbary GA2, Basha M1, Awad GE3, El-Hashemy HA1. J Liposome Res. 2016 Apr 15:1-42. [Epub ahead of print]
The current investigation aims to develop and evaluate novel ocular proniosomal gels of Lomefloxacin HCl (LXN); in order to improve its ocular bioavailability for the management of bacterial conjunctivitis. Proniosomes were prepared using different types of nonionic surfactants solely and as mixtures with Span 60. The formed gels were characterized for entrapment efficiency, vesicle size and in vitro drug release. Only Span 60 was able to form stable LXN proniosomal gel when used individually while the other surfactants formed gels only in combination with Span 60 at different ratios. The optimum proniosomal gel; P-LXN 7 (Span60:Tween60, 9:1) appeared as spherical shaped vesicles having high entrapment efficiency (>80%), appropriate vesicle size (187 nm) as well as controlled drug release over 12h. Differential scanning calorimetry confirmed the amorphous nature of LXN within the vesicles. Stability study did not show any significant changes in entrapment efficiency or vesicle size after storage for 3 months at 4°C.
4.Validation of the TrichinEasy® digestion system for the detection of Anisakidae larvae in fish products.
Cammilleri G, Chetta M, Costa A, Graci S, Collura R, Buscemi MD, Cusimano M, Alongi A, Principato D, Giangrosso G, Vella A, Ferrantelli V. Acta Parasitol. 2016 Jun 1;61(2):369-75. doi: 10.1515/ap-2016-0048.
Anisakis and other parasites belonging to the Anisakidae family are organisms of interest for human health, because of their high zoonotic potential. Parasites belonging to this family can cause Anisakiasis, a parasitological disease caused by the ingestion of raw, infested fish products. Furthermore, evidence from the EFSA (European Food Safety Authority; EFSA 2010) has highlighted the allergological potential of nematodes belonging to the Anisakis genre. The detection and identification of Anisakidae larvae in fish products requires an initial visual inspection of the fish sample, as well as other techniques such as candling, UV illumination and artificial digestion. The digestion method consists of the simulation of digestive mechanics, which is made possible by the utilization of HCl and pepsin, according to EC Regulation 2075/2005. In this study, a new Anisakidae larvae detection method using a mechanical digestion system called Trichineasy® was developed.