1. Synthesis, DNA binding and cleavage studies of Ni(II) complexes with fused aromatic N-containing ligands
C N Sudhamani, H S Bhojya Naik, T R Ravikumar Naik, M C Prabhakara Spectrochim Acta A Mol Biomol Spectrosc. 2009 Apr;72(3):643-7. doi: 10.1016/j.saa.2008.11.025. Epub 2008 Nov 27.
The three Ni(II) complexes of fused aromatic N-containing ligands such as [Ni(bnp)(3)](PF(6))(2) (1), [Ni(phen)(2)(bnp)](PF(6))(2) (2) and [Ni(bpy)(2)(bnp)](PF(6))(2) (3) (where bnp=dibenzo(b)1,8-naphthpyridine, phen=1,10-phenanthroline and bpy=bipyridine) were synthesized and structurally characterized. Elemental analysis, magnetic and spectroscopic data suggested octahedral geometry for all the complexes. Binding of these complexes with (ds)DNA were analyzed by absorption spectra, viscosity and thermal denaturation studies. Detailed analysis revealed that the metal complexes intercalates into the DNA base stack as intercalator. The oxidative cleavage activities of the complexes were studied with supercoiled (SC)pUC19 DNA by using gel electrophoresis, and the results show that complexes have potent nuclease activity.
2. Synthesis, DNA binding, and cleavage studies of Co(III) complexes with fused aromatic NO/NN-containing ligands
Chittanahalli N Sudhamani, Halehatty S Bhojya Naik, D Girija Nucleosides Nucleotides Nucleic Acids. 2012;31(2):130-46. doi: 10.1080/15257770.2011.644369.
Four new Co(III) complexes, namely [Co(cq)(3)](PF(6))(3), [Co(phen)(2)(cq)](PF(6))(3), [Co(bnp)(3)] (PF(6))(3), and [Co(phen)(2)(bnp)](PF(6))(3) (where cq = chromeno[2,3-b]quinoline, phen = 1,10-phenanthroline and bnp = dibenzo[b,g][1,8]naphthyridine), were synthesized and structurally characterized. Spectroscopic data suggested an octahedral geometry for all the complexes. Binding studies of these complexes with double-stranded (ds)DNA were analyzed by absorption spectra, viscosity, and thermal denaturation studies. The results revealed that the metal complex intercalates into the DNA base stack as intercalator. The oxidative cleavage activities of the complexes were studied with supercoiled pUC19 DNA using gel electrophoresis and the results show that the complexes have potent nuclease activity.
3. Synthesis, DNA-binding, DNA-photonuclease profiling and antimicrobial activity of novel tetra-aza macrocyclic Ni(II), Co(II) and Cu(II) complexes constrained by thiadiazole
B Vinay Kumar, H S Bhojya Naik, D Girija, N Sharath, S M Pradeepa, H Joy Hoskeri, M C Prabhakara Spectrochim Acta A Mol Biomol Spectrosc. 2012 Aug;94:192-9. doi: 10.1016/j.saa.2012.03.071. Epub 2012 Mar 29.
A new tetra-aza macrocyclic ligand, L (C(24)H(16)N(12)O(2)S(4)) and its complexes of type, [MLCl(2)] and [CuL]Cl(2) (where M=Ni(II), Co(II); L=N,N'-(benzene-1,3-diyldi-1,3,4-thiadiazole-5,2-diyl)bis{2-[(5-benzene-1,3-diyl-1,3,4-thiadiazol-2-yl)amino]acetamide}) were synthesized and characterized by the spectral and analytical techniques. An octahedral geometry has been proposed for Ni(II) and Co(II) complexes while Cu(II) complex exhibit a square planar geometry. All the synthesized metal complexes were screened for their in vitro antimicrobial activity against selected species of pathogenic bacteria and fungi. The binding property of the complexes with CT-DNA was studied by absorption spectral analysis, followed by viscosity measurement and thermal denaturation studies. The photo induced cleavage studies revealed that the complexes possess photonuclease property against pUC19 DNA under UV-visible irradiation.
![Fmoc-Leu-OH-[15N]](https://resource.bocsci.com/structure/200937-57-1.gif)
