Fmoc-L-glutamic acid γ-tert-butyl ester α-N-hydroxysuccinimide ester
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Fmoc-L-glutamic acid γ-tert-butyl ester α-N-hydroxysuccinimide ester

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Category
Fmoc-Amino Acids
Catalog number
BAT-003762
CAS number
101214-22-6
Molecular Formula
C28H30N2O6
Molecular Weight
522.55
Fmoc-L-glutamic acid γ-tert-butyl ester α-N-hydroxysuccinimide ester
IUPAC Name
5-O-tert-butyl 1-O-(2,5-dioxopyrrolidin-1-yl) (2S)-2-(9H-fluoren-9-ylmethoxycarbonylamino)pentanedioate
Synonyms
Fmoc-L-Glu(OtBu)-OSu; (S)-5-tert-Butyl 1-(2,5-dioxopyrrolidin-1-yl)2-((((9H-fluoren-9-yl)methoxy)carbonyl)amino)pentanedioate
Appearance
White powder
Purity
≥ 99% (HPLC)
Density
1.34±0.1 g/cm3
Melting Point
159-166 °C
Storage
Store at 2-8 °C
InChI
InChI=1S/C28H30N2O8/c1-28(2,3)37-25(33)15-12-22(26(34)38-30-23(31)13-14-24(30)32)29-27(35)36-16-21-19-10-6-4-8-17(19)18-9-5-7-11-20(18)21/h4-11,21-22H,12-16H2,1-3H3,(H,29,35)/t22-/m0/s1
InChI Key
TUXJIYSGLYYEHH-QFIPXVFZSA-N
Canonical SMILES
CC(C)(C)OC(=O)CCC(C(=O)ON1C(=O)CCC1=O)NC(=O)OCC2C3=CC=CC=C3C4=CC=CC=C24

Fmoc-L-glutamic acid γ-tert-butyl ester α-N-hydroxysuccinimide ester is a modified derivative of L-glutamic acid, where the amino group is protected by a 9-fluorenylmethoxycarbonyl (Fmoc) group, the γ-carboxyl group is esterified with a tert-butyl group, and the α-carboxyl group is linked to a N-hydroxysuccinimide (NHS) ester. The NHS ester functionality makes this compound highly reactive toward primary amines, enabling it to be used in coupling reactions during peptide synthesis. The Fmoc group allows selective deprotection under basic conditions, making it useful in stepwise peptide assembly.

One of the key applications of Fmoc-L-glutamic acid γ-tert-butyl ester α-N-hydroxysuccinimide ester is in peptide synthesis, particularly in creating peptides with glutamic acid modifications. The NHS ester is highly reactive with amines, making it ideal for coupling reactions in peptide chemistry. The Fmoc protection on the amino group allows for controlled deprotection during the peptide elongation process. This combination of functionalities allows for efficient and selective incorporation of modified glutamic acid residues into peptides, which is useful for generating peptides with specific properties for research and drug development.

Another significant application of this compound is in the synthesis of peptide conjugates. The NHS ester can be used to attach peptides to other molecules, such as drugs, biomolecules, or nanoparticles, facilitating the creation of conjugates for targeted drug delivery. By reacting the NHS ester with a variety of primary amines, researchers can selectively conjugate peptides to carriers or therapeutic agents, allowing for the development of more effective and targeted therapies. This approach is particularly valuable in cancer therapy, where selective delivery of cytotoxic drugs to tumor cells is critical.

Fmoc-L-glutamic acid γ-tert-butyl ester α-N-hydroxysuccinimide ester is also employed in the design of novel materials, including polymeric systems for drug delivery. The ability to incorporate this compound into polymers enables the creation of biocompatible materials capable of controlled drug release. The NHS ester reacts with amine-functionalized molecules, making it useful in the preparation of peptide-functionalized polymers or hydrogels that can encapsulate and release drugs in a controlled manner. These materials can improve the pharmacokinetics of drugs and allow for sustained release, enhancing their therapeutic effectiveness.

Finally, Fmoc-L-glutamic acid γ-tert-butyl ester α-N-hydroxysuccinimide ester is useful in the development of antibody-drug conjugates (ADCs). The NHS ester can be used to link therapeutic peptides to antibodies, creating conjugates that specifically target antigens on cancer cells. This targeted delivery system minimizes the toxicity to healthy tissues and maximizes the effectiveness of the drug at the site of action. By employing this compound, researchers can generate ADCs with improved selectivity and therapeutic potential, providing a promising approach for cancer treatment and other diseases.

1. Enhanced stereoselectivity of a Cu(II) complex chiral auxiliary in the synthesis of Fmoc-L-γ-carboxyglutamic acid
Daniel J Smith, Glenn P A Yap, James A Kelley, Joel P Schneider J Org Chem. 2011 Mar 18;76(6):1513-20. doi: 10.1021/jo101940k. Epub 2011 Feb 3.
L-γ-Carboxyglutamic acid (Gla) is an uncommon amino acid that binds avidly to mineral surfaces and metal ions. Herein, we report the synthesis of N-α-Fmoc-L-γ-carboxyglutamic acid γ,γ'-tert-butyl ester (Fmoc-Gla(O(t)Bu)(2)-OH), a suitably protected analogue for Fmoc-based solid-phase peptide synthesis. The residue was synthesized using a novel chiral Cu(II) complex, whose structure-based design was inspired by the blue copper protein rusticyanin. The five-coordinate complex is formed by Shiff base formation between glycine and the novel ligand (S)-2-(N-(2-methylthio)benzylprolyl)aminobenzophenone in the presence of copper. Michael addition of di-tert-butyl methylenemalonate to the α-carbon of the glycine portion of the complex occurs in a diastereoselective fashion. The resulting (S,S)-complex diastereomer can be easily purified by chromatography. Metal complex decomposition followed by Fmoc protection affords the enantiomerically pure amino acid. With the use of this novel chiral complex, the asymmetric synthesis of Fmoc-Gla(O(t)Bu)(2)-OH was completed in nine steps from thiosalicylic acid in 14.5% overall yield.
2. Methotrexate analogues. 31. Meta and ortho isomers of aminopterin, compounds with a double bond in the side chain, and a novel analogue modified at the alpha-carbon: chemical and in vitro biological studies
A Rosowsky, H Bader, R A Forsch, R G Moran, J H Freisheim J Med Chem. 1988 Apr;31(4):763-8. doi: 10.1021/jm00399a013.
Five heretofore undescribed analogues of methotrexate (MTX) and aminopterin (AMT) were synthesized and tested as dihydrofolate reductase (DHFR) inhibitors and tumor cell growth inhibitors. The meta isomer of AMT was obtained from 2,4-diamino-6-(bromomethyl)pteridine and m-(aminobenzoyl)-L-glutamic acid, while the ortho isomer was obtained via the same route by using alpha-methyl gamma-tert-butyl o-(aminobenzoyl)-L-glutamate instead of the free acid. Analogues of MTX and AMT containing a double bond in the side chain were prepared from dimethyl D,L-2-amino-4-hexenedioate and 4-amino-4-deoxy-N10-methylpteroic acid and 4-amino-4-deoxy-N10-formylpteroic acid, respectively. Finally, a positional isomer of MTX with the CH2CH2COOH moiety moved from the alpha-carbon to the adjacent carboxamide nitrogen was synthesized from 3-[N-(carboxymethyl)amino]propanoic acid diethyl ester and 4-amino-4-deoxy-N10-methylpteroic acid. The positional isomers of AMT were weak DHFR inhibitors and showed very little growth-inhibitory activity against L1210 murine leukemia cells or the MTX-resistant L1210/R81 mutant line in culture. The MTX and AMT analogues with the CH2CH2COOH moiety replaced by a CH2CH = CHCOOH side chain showed anti-DHFR activity similar to that of the previously described saturated compound N-(4-amino-4-deoxy-N10-methylpteroyl)-L-2-aminoadipic acid, but were less potent than the parent drugs. The MTX analogue with the CH2CH2COOH side chain displaced from C to N was weakly bound to DHFR, confirming the importance of an intact CONH moiety, and showed greatly diminished cell growth inhibitory potency relative to MTX. None of the compounds was a substrate for folylpolyglutamate synthetase (FPGS) from mouse liver. Furthermore, inhibition of folic acid polyglutamylation in vitro at equimolar 500 microM concentrations of drug and substrate was negligible. The structural changes embodied in these five novel compounds are therefore too great for binding to the FPGS active site.
3. Discovery of new targeting agents against GAPDH receptor for antituberculosis drug delivery
Muhammad Amirul Asyraf Noh, Siti Sarah Fazalul Rahiman, Habibah A Wahab, Amirah Mohd Gazzali J Basic Clin Physiol Pharmacol. 2021 Jun 25;32(4):715-722. doi: 10.1515/jbcpp-2020-0435.
Objectives: Tuberculosis (TB) remains a public health concern due to the emergence and evolution of multidrug-resistant strains. To overcome this issue, reinforcing the effectiveness of first line antituberculosis agents using targeted drug delivery approach is an option. Glyceraldehyde-3-Phosphate Dehydrogenase (GADPH), a common virulence factor found in the pathogenic microorganisms has recently been discovered on the cell-surface of Mycobacterium tuberculosis, allowing it to be used as a drug target for TB. This study aims to discover active small molecule(s) that target GAPDH and eventually enhance the delivery of antituberculosis drugs. Methods: Ten ligands with reported in vitro and/or in vivo activities against GAPDH were evaluated for their binding interactions through molecular docking studies using AutoDock 4.2 program. The ligand with the best binding energy was then modified to produce 10 derivatives, which were redocked against GAPDH using previous protocols. BIOVIA Discovery Studio Visualizer 2019 was used to explore the ligand-receptor interactions between the derivatives and GAPDH. Results: Among the 10 ligands, curcumin, koningic acid and folic acid showed the best binding energies. Further analysis on the docking of two folic acid derivatives, F7 (γ-{[tert-butyl-N-(6-aminohexyl)]carbamate}folic acid) and F8 (folic acid N-hydroxysuccinimide ester) showed that the addition of a bulky substituent at the carboxyl group of the glutamic acid subcomponent resulted in improved binding energy. Conclusions: Folic acid and the two derivatives F7 and F8 have huge potentials to be developed as targeting agents against the GAPDH receptor. Further study is currently on-going to evaluate the effectiveness of these molecules in vitro.
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