Inquiry Basket
Fmoc-S-3-nitro-2-pyridine-sulfenyl-L-cysteine, a specialized reagent pivotal in peptide synthesis and protein engineering.
Solid-Phase Peptide Synthesis: Employing this compound is essential for building peptide chains through solid-phase techniques. The Fmoc-protecting group enables the sequential addition of amino acids, facilitating the synthesis of intricate peptides. With the sulfenyl group warding off unwanted side reactions, the desired peptide product is obtained with superior purity and yield.
Protein Engineering: Delving into protein modification, Fmoc-S-3-nitro-2-pyridine-sulfenyl-L-cysteine allows for targeted alterations on cysteine residues within proteins. This avenue empowers researchers to explore the impact of modifications on protein structure and function, paving the way for proteins with enhanced stability, modified activity, or novel characteristics for diverse therapeutic and industrial applications.
Bioorthogonal Chemistry: In the realm of bioorthogonal labeling and conjugation, this compound presents a gateway to incorporating functional groups capable of selective chemical reactions. This facility enables the tagging of peptides and proteins with various labels, such as fluorophores or affinity tags, sans disruption to native biomolecular functions. Such cutting-edge techniques play a vital role in advanced imaging, diagnostics, and therapeutic applications.
Disulfide Bond Formation Studies: Aiding in the exploration of disulfide bond dynamics, Fmoc-S-3-nitro-2-pyridine-sulfenyl-L-cysteine is instrumental in dissecting disulfide bond formation and isomerization within peptides and proteins. By integrating this compound, researchers can systematically create and analyze disulfide bonds, unraveling their significance in protein folding and stability. This knowledge contributes to the development of proteins with finely tuned disulfide linkages, crucial for pharmaceutical formulations.
