1.[Experience in investigating splenic red pulp lymphoma].
Al'-Radi LS1, Moiseeva TN1, Dzhulakyan UL1, Danishyan KI1, Kovrigina AM1, Glebova SM1, Lugovskaya SA2, Dvirnyk VN1, Khvastunova AN3, Yakutik IA1, Savchenko VG1. Ter Arkh. 2016;88(4):53-60.
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English, RussianРезюме Цель исследования. Обобщить опыт гематологов по диагностике и дифференциальной диагностике лимфомы красной пульпы селезенки (ЛКПС). Материалы и методы. За 2013—2014 гг. в ФГБУ ГНЦ выполнено исследование биоптатов 87 селезенок, удаленных при различных В-клеточных лимфопролиферативных заболеваниях. В 4 (4,6%) случаях на основании морфологического, иммуногистохимического, иммунофенотипического, молекулярного исследований биоптата селезенки, проб крови, костного мозга (КМ) установлен диагноз ЛКПС. Результаты. Во всех случаях ЛКПС наблюдалась значительная спленомегалия, лимфоцитоз от 56 до 94% (в 2 случаях с лейкоцитозом 55·109 и 75·109/л), циркуляция «ворсинчатых» лимфоцитов с фенотипом CD20+(ярко), CD11c+/±, CD103(слабо)+/±, LAIR-1+, CD25–, CD5–, CD10–, CD23–, не содержащих тартратустойчивую кислую фосфатазу, без мутации BRAFV600E. КМ с незначительной лимфоидной инфильтрацией CD20+, CD25–, Annexin 1–, Cyclin D1–.
2.Controlled Inactivation of Trichophyton rubrum Using Shaped Electrical Pulse Bursts: Parametric Analysis.
Novickij V1, Grainys A1, Švedienė J2, Paškevičius A2,3, Novickij J1. Biotechnol Prog. 2016 Apr 12. doi: 10.1002/btpr.2276. [Epub ahead of print]
The dermatophytes infect the skin by adherence to the epidermis followed by germination, growth and penetration of the fungal hyphae within the cells. The aim of this study was to investigate the efficacy of the pulsed electric fields (PEF) of controlled inactivation of Trichophyton rubrum (ATCC 28188). In this work we have used bursts of the square wave PEF pulses of different intensity (10-30 kV/cm) to induce the irreversible inactivation in vitro. The electric field pulses of 50 µs and 100 µs have been generated in bursts of 5, 10 and 20 pulses with repetition frequency of 1 Hz. The dynamics of the inactivation using different treatment parameters were studied and the inactivation map for the T. rubrum has been defined. Further, the combined effect of pulsed electric fields with the antifungal agents itraconazole, terbinafine and naftifine HCl was investigated. It has been demonstrated that the combined effect results in the full inactivation of T.
3.Intravaginal administration of metformin hydrochloride loaded cationic niosomes amalgamated with thermosensitive gel for the treatment of polycystic ovary syndrome: In vitro and in vivo studies.
Saini N1, Sodhi RK2, Bajaj L1, Pandey RS3, Jain UK1, Katare OP4, Madan J5. Colloids Surf B Biointerfaces. 2016 Apr 8;144:161-169. doi: 10.1016/j.colsurfb.2016.04.016. [Epub ahead of print]
BACKGROUND AND OBJECTIVE: Metformin hydrochloride (MTF-HCl) is extensively recommended by physicians for the treatment of polycystic ovary syndrome (PCOS). Mechanistically, MTF-HCl activates AMP-dependent kinase-α (AMPK-α) pathway to decrease the glucose production, enhances fatty acid oxidation and elevates the uptake of glucose in tissues. However, despite favourable physicochemical properties, oral administration of MTF-HCl is associated with impaired bioavailability (50-60%), lactic-acidosis and frequent dosing (500mg 2-3 times a day) in PCOS that ultimately influence the patient compliance. Therefore, in present investigation, MTF-HCl loaded unmodified and cationic small unilamellar niosomes were separately amalgamated with thermosensitive gel (MTF-HCl-SUNs-Gel and MTF-HCl-C-SUNs-Gel) for the treatment of PCOS through vaginal route of administration.
4.Mucosal acidification increases hydrogen sulfide release through up-regulating gene and protein expressions of cystathionine gamma-lyase in the rat gastric mucosa.
Mard SA1, Veisi A2, Ahangarpour A2, Gharib-Naseri MK2. Iran J Basic Med Sci. 2016 Feb;19(2):172-7.
OBJECTIVES: This study was performed to investigate the effects of mucosal acidification on mRNA expression and protein synthesis of cystathionine gamma lyase (CSE), cystathionine beta synthase (CBS), and mucosal release of H2S in gastric mucosa in rats.
