L-Asparagine 4-nitrobenzyl ester 4-toluenesulfonate salt
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L-Asparagine 4-nitrobenzyl ester 4-toluenesulfonate salt

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Category
L-Amino Acids
Catalog number
BAT-003962
CAS number
3561-57-7
Molecular Formula
C11H13N3O5·HBr
Molecular Weight
348.15
L-Asparagine 4-nitrobenzyl ester 4-toluenesulfonate salt
IUPAC Name
(4-nitrophenyl)methyl 2,4-diamino-4-oxobutanoate;hydrobromide
Synonyms
L-Asn-ONb TosOH; (4-nitrophenyl)methyl 2,4-diamino-4-oxobutanoate,hydrobromide; Asn-Onb HBr
Appearance
Off-white powder
Purity
≥ 98% (TLC)
Density
1.392 g/cm3
Melting Point
166-170 °C
Boiling Point
529.7 °C at 760 mmHg
Storage
Store at 2-8 °C
InChI
InChI=1S/C11H13N3O5.BrH/c12-9(5-10(13)15)11(16)19-6-7-1-3-8(4-2-7)14(17)18;/h1-4,9H,5-6,12H2,(H2,13,15);1H
InChI Key
GHBAUQLEMIMDES-UHFFFAOYSA-N
Canonical SMILES
C1=CC(=CC=C1COC(=O)C(CC(=O)N)N)[N+](=O)[O-].Br

L-Asparagine 4-nitrobenzyl ester 4-toluenesulfonate salt, a versatile compound with diverse applications in bioscience and chemistry, plays a pivotal role in various fields. Here are four key applications presented with high perplexity and burstiness:

Peptide Synthesis: An essential component in peptide synthesis, L-Asparagine 4-nitrobenzyl ester 4-toluenesulfonate salt acts as a fundamental building block. Its ester form facilitates the intricate process of peptide bond formation during solid-phase peptide synthesis. This capability is crucial for the production of tailored peptides for both research and therapeutic applications, including the advancement of peptide-based pharmaceuticals.

Enzyme Substrate Studies: Serving as a substrate in enzyme assays, this compound is invaluable for investigating the activities of specific proteases and amidases. By monitoring the cleavage of the ester bond, researchers can delve into enzyme kinetics and inhibitor effectiveness. This knowledge is pivotal in drug discovery endeavors and the comprehensive understanding of enzyme functionalities, adding depth to biochemical research.

Proteomics: Within the realm of proteomics, L-Asparagine 4-nitrobenzyl ester 4-toluenesulfonate salt finds utility in protein modification and labeling. This compound allows for the integration of specific tags that enhance the detection and quantification of proteins within intricate samples. Such methodology is vital for protein profiling, the study of protein interactions, and unraveling the complexities of cellular proteomes, shedding light on the intricate web of cellular processes.

Chemical Biology: In the expansive domain of chemical biology, this compound serves as a crucial tool for synthesizing chemical probes and exploring biological pathways. By conjugating this compound with bioactive molecules, researchers can track the interactions and distribution patterns of these molecules within cellular environments. This innovative approach aids in elucidating biological mechanisms and pinpointing potential targets for therapeutic intervention, pushing the boundaries of biological research.

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