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Application Area

L-Aspartic acid diethyl ester hydrochloride

* Please kindly note that our products are not to be used for therapeutic purposes and cannot be sold to patients.

Category

β−Amino Acids

Catalog number

BAT-004223

CAS number

16115-68-7

Molecular Formula

C8H15NO4·HCl

Molecular Weight

225.62

L-Aspartic acid diethyl ester hydrochloride

IUPAC Name

diethyl (2S)-2-aminobutanedioate;hydrochloride

Synonyms

L-Asp(OEt)-OEt HCl; H-ASP(OET)-OET HCl; H-Asp(OEt)-OEt hydrochloride; Diethyl L-Aspartate Hydrochloride; L-Aspartic Acid Diethyl Ester Hydrochloride

Appearance

White powder

Purity

≥ 99% (HPLC)

Density

1.102 g/cm3

Melting Point

106-111 °C

Boiling Point

259.5 °C at 760 mmHg

Storage

Store at 2-8 °C

InChI

InChI=1S/C8H15NO4.ClH/c1-3-12-7(10)5-6(9)8(11)13-4-2;/h6H,3-5,9H2,1-2H3;1H/t6-;/m0./s1

InChI Key

AJOXZAAREAYBQR-RGMNGODLSA-N

Canonical SMILES

CCOC(=O)CC(C(=O)OCC)N.Cl

L-Aspartic acid diethyl ester hydrochloride is a chemical compound derived from aspartic acid, an amino acid found naturally in the human body and various foods. It is an esterified form, where the carboxyl groups of aspartic acid are converted into ester groups by introducing ethyl groups. This modification makes the compound more lipophilic, allowing it to cross biological membranes more easily than its parent amino acid. The hydrochloride is a salt form which improves the compound’s stability and solubility in aqueous solutions. L-Aspartic acid diethyl ester hydrochloride is synthesized through an esterification process involving aspartic acid and ethanol, followed by a reaction with hydrochloric acid. This chemical is typically used in research environments for its unique properties, serving as a building block in synthesizing various compounds.

One key application of L-Aspartic acid diethyl ester hydrochloride is in pharmaceutical research, where it acts as a versatile intermediate in drug synthesis. Because of its modified ester structure, it can be utilized to develop prodrugs that require enhanced cellular uptake. Prodrugs are inactive compounds that metabolize into active drugs once they enter the body. By leveraging the compound’s ester features, researchers can create medications that have improved bioavailability and efficiency. This property is particularly useful in developing treatments for neurological disorders, where crossing the blood-brain barrier effectively is often a significant challenge.

Another important application of L-Aspartic acid diethyl ester hydrochloride is in peptide synthesis. The compound is used to protect amino acid sequences during the process of forming peptide chains. Its ester groups prevent undesirable reactions at the carboxyl sites, allowing chemists to selectively deprotect and couple peptides with greater control and efficiency. This aspect is crucial in biochemical research and industrial production of peptides, where precision is essential to ensure the biological activity and function of the synthesized peptides.

L-Aspartic acid diethyl ester hydrochloride also finds usage in agricultural chemistry, particularly in the formulation of bioactive compounds that can enhance plant growth and stress resistance. The compound’s ability to facilitate the synthesis of agrichemicals that penetrate plant tissues more efficiently allows for the development of novel fertilizers and growth enhancers. These products can improve nutrient absorption and bolster plants’ resilience against environmental stressors such as drought or disease, thus contributing to more sustainable agricultural practices.

In the field of material science, L-Aspartic acid diethyl ester hydrochloride is utilized to create polymeric materials with enhanced structural and chemical properties. By incorporating this compound into polymer matrices, researchers can develop materials with improved flexibility, strength, and resistance to environmental degradation. Such materials are valuable in creating advanced coatings, adhesives, and biomedical devices. The ester’s reactive nature allows it to form cross-linked structures within polymers, thereby enhancing their durability and performance. This application underscores the compound’s versatility and its potential in generating innovative materials for various industrial uses.

1. Effects of onopordia, a novel isolated compound from Onopordon acanthium, on pentylenetetrazole-induced seizures in mice: Possible involvement of nitric oxide pathway

Malihe Hassanzadeh, Niusha Sharifi, Shabnam Mahernia, Nastaran Rahimi, Ahmad Reza Dehpour, Massoud Amanlou J Tradit Complement Med. 2019 Nov 30;11(1):22-26. doi: 10.1016/j.jtcme.2019.11.005. eCollection 2021 Jan.

Epilepsy is identified as a brain disorder and characterized by unpredictable disruption of normal brain function. Due to adverse side effect associated with antiepileptic drugs and also resistance profile, improvement of antiepileptic medications with more beneficial anticonvulsant activity is essential. Natural products have demonstrated their therapeutic properties such as anxiolytic, antidepressant and anticonvulsant activities and a source for identification of novel lead compounds. Therefore, the purpose of this study was to evaluate the effects of Onopordon acanthium secondary metabolite, onopordia, on pentylenetetrazole (PTZ)-induced seizure in male mice and investigate the possible role of nitric oxide pathway. Different doses of onopordia (0.1, 1 and 10 mg/kg) and phenobarbital (20 mg/kg) were administered intraperitoneally (i.p., 30, 60 and 120 min) prior to induction of epileptic seizure and compared to control groups. Onopordia demonstrated anticonvulsant effects when administrated at dose of 10 mg/kg, i.p. and optimum time 60 min prior to induction of seizure. Anticonvulsant effect of onopordia was blocked by applying a single dose of a non-selective nitric oxide synthase (NOS) inhibitor, Nω-nitro-l-arginine methyl ester hydrochloride (l-NAME; 10 mg/kg, i.p.), and also a single dose of a selective neuronal NOS (nNOS) inhibitor, 7-nitroindazole (7-NI; 30 mg/kg, i.p.). Administration of ketamine as a N-Methyl-d-aspartic acid (NMDA) receptor antagonist (0.5 mg/kg; i.p.) with onopordia did not change the anticonvulsant effect of onopordia. The results of the present study demonstrated the anticonvulsant effect of onopordia as a new lead compound and also contribution of NO/nNOS pathway on PTZ-induced seizure in mice.

2. Effects of dibenzylbutyrolactone lignans arctigenin and trachelogenin on the motility of isolated rat ileum

Peter Kiplang'at Koech, Imre Boldizsár, Arpád Dobolyi, Petra Varró Toxicol Rep. 2022 May 27;9:1222-1232. doi: 10.1016/j.toxrep.2022.05.019. eCollection 2022.

Dibenzylbutyrolactone-type lignans are phenolic compounds of medical importance. The purpose of the study was to determine the effects of two such lignans, arctigenin and trachelogenin on the motility of isolated rat ileum and obtain indications on their mechanism of action. They were isolated from Arctium lappa and Cirsium arvense, respectively, which have been used traditionally to treat gastrointestinal disorders. 1-1.5 cm long segments of distal ileum were obtained from adult male Wistar rats. The intestinal segments were suspended vertically in a well-aerated organ-bath according to Magnus mounting method. The intestinal motility was monitored for 30 min before treatment to obtain the baseline, followed by treatment with 1 µM, 10 µM, 20 µM and 40 µM concentrations of arctigenin and 0.5 µM, 1 µM, 10 µM and 20 µM of trachelogenin concentrations. The amplitude, tone, and period of spontaneous contractions were measured after 15 and 30 min of treatment. To investigate their mechanism of action, cholinergic, glutamatergic, adrenergic antagonists and compounds inhibiting nitric oxide synthase and L-type calcium channels were also tested. Arctigenin and trachelogenin decreased the frequency of contractions in a dose-dependent manner. At the concentration of 20 µM and 40 µM of trachelogenin and arctigenin, respectively, there was a marked alteration in spontaneous contraction pattern with an observable increase in the period time. This activity was comparable to 0.5 µM nifedipine (L-type calcium channel blocker) treatment. Our results demonstrate relaxant effect of arctigenin and trachelogenin on the ileum motility that may be mediated by L-type calcium ion channel blockade.

3. Nitric oxide regulates the firing rate of neuronal subtypes in the guinea pig ventral cochlear nucleus

Adam Hockley, Joel I Berger, Paul A Smith, Alan R Palmer, Mark N Wallace Eur J Neurosci. 2020 Feb;51(4):963-983. doi: 10.1111/ejn.14572. Epub 2019 Oct 10.

The gaseous free radical, nitric oxide (NO) acts as a ubiquitous neuromodulator, contributing to synaptic plasticity in a complex way that can involve either long term potentiation or depression. It is produced by neuronal nitric oxide synthase (nNOS) which is presynaptically expressed and also located postsynaptically in the membrane and cytoplasm of a subpopulation of each major neuronal type in the ventral cochlear nucleus (VCN). We have used iontophoresis in vivo to study the effect of the NOS inhibitor L-NAME (L-NG-Nitroarginine methyl ester) and the NO donors SIN-1 (3-Morpholinosydnonimine hydrochloride) and SNOG (S-Nitrosoglutathione) on VCN units under urethane anaesthesia. Collectively, both donors produced increases and decreases in driven and spontaneous firing rates of some neurones. Inhibition of endogenous NO production with L-NAME evoked a consistent increase in driven firing rates in 18% of units without much effect on spontaneous rate. This reduction of gain produced by endogenous NO was mirrored when studying the effect of L-NAME on NMDA(N-Methyl-D-aspartic acid)-evoked excitation, with 30% of units showing enhanced NMDA-evoked excitation during L-NAME application (reduced NO levels). Approximately 25% of neurones contain nNOS and the NO produced can modulate the firing rate of the main principal cells: medium stellates (choppers), large stellates (onset responses) and bushy cells (primary-like responses). The main endogenous role of NO seems to be to partly suppress driven firing rates associated with NMDA channel activity but there is scope for it to increase neural gain if there were a pathological increase in its production following hearing loss.