1. Comparative bioefficacy of lysine from l-lysine hydrochloride or l-lysine sulfate in basal diets containing graded levels of canola meal for female broiler chickens.
M Aslam Mirza, T Mushtaq, Z Ahmed, G Ahmad. Poult Sci. 2007 Mar; 86(3): 525-30. DOI: 10.1093/ps/86.3.525. PMID: 17297165.
A broiler growth assay was conducted to compare the efficacy of l-lysine HCl and l-lysine sulfate at a graded addition of canola meal (CM). A total of 1,440 1-d-old female Hubbard broiler chicks were allotted randomly to 6 dietary treatments each in 4 replicates of 60 birds per pen. The 2 lysine sources (l-lysine HCl and l-lysine sulfate) and the 3 CM levels (10, 15, and 20%) were used in 2 x 3 factorial arrangement in isonitrogenous (19% CP) and equicaloric (2,700 kcal of ME/kg) diets containing 0.96% digestible lysine. The experiment lasted for 42 d, and a single mash diet was used throughout the experiment. The feed intake during the starter phase (1 to 28 d) decreased linearly as the dietary CM level increased with diets containing l-lysine HCl, whereas feed intake increased linearly with increasing dietary CM level with that of lysine sulfate. Gizzard weight as percentage of carcass weight increased linearly (P < or = 0.016) as dietary CM level increased. No significant effect of lysine sources or CM was observed on body weight gain, feed:gain, mortality, carcass weight, breast and thigh yield, and abdominal fat. In conclusion, l-lysine HCl can be replaced with l-lysine sulfate for broiler diets, and CM can be used as up to 20% of the starter (1 to 28 d) and finisher (29 to 42 d) diets without having any adverse effects of broiler performance.
2. Development and validation of new hplc method for simultaneous estimation of l-lysine hydrochloride and l-carnitine-l-tartrate in pharmaceutical dosage form.
M Ahmed, M A Qadir, W A Hussain, M S Tahir. Indian J Pharm Sci. Jul-Aug 2015; 77(4): 434-8. DOI: 10.4103/0250-474x.164772. PMID: 26664059.
A new analytical method is developed and validates for simultaneous estimation of L-lysine hydrochloride and L-carnitine-L-tartrate in single pharmaceutical dosage form by means of high resolution HPLC. The chromatographic procedure was carried out using C18-5 μm (4.6 mm × 250 mm), column. Optimally suited mobile phase was made to run in an isocratic elution mode whose composition was 10 mM of potassium dihydrogen phosphate adjusted with triethylamine to a pH of 7.5 and pumped at a flow rate of 0.50 ml/min through chromatographic system. The detector's wavelength was 214 nm. The accuracy of the analytical method was determined keeping into account the recovery of analytes, which in this case remains well within the accepted standards that is 95-105%. Detection limit for L-lysine hydrochloride and L-carnitine-L-tartrate are 1.47 μg/ml and 0.85 μg/ml while quantitation limit for L-lysine hydrochloride and L-carnitine-L-tartrate is 4.41 and 2.55 μg/ml, respectively. All the statistical results were validated performing precision, accuracy, linearity, specificity studies for analytes concentration from 70-130%. The outcome of results confirmed that the method was in consonance with the acceptance criteria.
