N-α-(9-Fluorenylmethoxycarbonyl)-L-α,γ-diaminobutyric acid

A Gram-staining-positive, heterotrophic, non-spore-forming, rod-shaped, strain OAct353T belonging to the genus Agromyces was isolated from a soil sample collected from a coastal wetland of the Yellow River delta, China. The strain was identified using a polyphasic taxonomic approach. The strain grew in the presence of 0-10% (w/v) NaCl (optimum, 2-3 %), at pH 5.0-8.0 (optimum, pH 7.0) and 12-36 °C (optimum, 28 °C). The isolate contained 2,4-diaminobutyric acid, glutamic acid and glycine in its peptidoglycan. The acyl type of the cell-wall muramic acid was N-acetyl. The whole cell sugars of this novel strain were glucose, xylose and rhamnose. The predominant menaquinones were MK-12 (74 %) and MK-11 (21 %). The major phospholipids were phosphatidylglycerol (PG), one unknown phospholipid (PL), three unknown glycolipids (GL) and three unknown polar lipids (L). The major fatty acids were iso-C16:0, anteiso-C15:0 and anteiso-C17:0. The DNA G+C content was 69.

A Gram-stain positive, aerobic, rod-shaped, non-motile actinomycete strain, designated EGI 60007T, was isolated from healthy roots of Glycyrrhiza uralensis F. collected from Yili County, Xinjiang Province, North-West China. A polyphasic approach was applied to study the taxonomic position of the new isolate. The 16S rRNA gene sequence of strain EGI 60007T had highest sequence similarities with members of the genus Pseudoclavibacter: Pseudoclavibacter chunganensis CAU59T (96.98%), Pseudoclavibacter helvolus DSM 20419T (96.43%) and Pseudoclavibacter terrae THG-MD12T (96.14%). The phylogenetic neighbour joining tree based on 16S rRNA gene sequences showed that strain EGI 60007T clustered with the genus Pseudoclavibacter, and formed a distinct clade with P. chungangensis CAU59T. The polar lipids detected for strain EGI 60007T were phosphatidylglycerol, diphosphatidylglycerol, one unidentified glycolipid and one unidentified lipid. The DNA G+C content was determined to be 63.

A novel Gram-reaction-positive, non-motile, aerobic bacterium, designated CFH S0483T, was isolated from a soil sample collected from Catba island in Halong Bay, Vietnam. 16S rRNA gene sequence analysis showed that the strain is a member of the genus Agromyces and it has higher similarities with Agromyces humatus DSM 16389T (97.3%) and Agromyces ramosus DSM 43045T (97.1%), and lower 97.0% with other Agromyces type strains. Strain CFH S0483T was able to grow at 10-37 oC, pH 7.0-9.0 and with a NaCl tolerance up to 2.0% (w/v). The whole-cell sugars were mannose, galactose, glucose and ribose. The isolate contained L-2,4-diaminobutyric acid, D-alanine, D-glutamic, and glycine in the cell-wall peptidoglycan. Strain CFH S0483T exhibited a menaquinone system with MK-12, and the major fatty acids were anteiso-C15:0, anteiso-C17:0 and iso-C16:0. The genomic DNA G+C content of strain CFH S0483T was 71.6 mol%. Based on the phylogenetic and phenotypic analysis, and low value of DNA-DNA hybridization, strain CFH S0483T could not be classified into any recognized species of the genus Agromyces.

Tomato style is the pathway for pollen germination and pollen tubes growth from the stigma to the ovules where fertilization occurs. It is essential to supplying the nutrients for pollen tube growth and guidance for the pollen tubes. To our knowledge, style also regulates gametophytic self-incompatibility (SI) in tomato species. This study identified the metabolites and monitored the metabolic changes of self-incompatible and self-compatible tomato with self-pollinated or unpollinated styles by gas chromatography-mass spectrometry (GC-MS). A total of 9 classes of compounds were identified in SI and self-compatibility (SC) self-pollinated and unpollinated styles which included amino acids, sugars, fatty acids/lipids, amines, organic acids, alcohols, nitriles, inorganic acids and other compounds. The contents of d-Mannose-6-phosphate, Cellobiose, Myristic acid, 2,4-Diaminobutyric acid, Inositol and Urea were significantly decreased and the rest did not significantly change in SI styles.