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A substrate for trypsin and papain.
Nα-Acetyl-L-arginine 4-nitroanilide hydrochloride is a biochemical reagent with a variety of specialized applications in bioscience. Here are some key applications of Nα-Acetyl-L-arginine 4-nitroanilide hydrochloride:
Enzyme Assays: Nα-Acetyl-L-arginine 4-nitroanilide hydrochloride is commonly used as a substrate in the measurement of arginase activity, an enzyme involved in the urea cycle. By quantifying the rate at which the substrate is converted by the enzyme, researchers can determine arginase activity in various biological samples. This is crucial for understanding metabolic disorders and for the development of enzyme inhibitors.
Clinical Diagnostics: This compound serves as a diagnostic reagent for determining arginine and arginase levels in clinical samples such as serum or urine. The straightforward colorimetric assay enabled by Nα-Acetyl-L-arginine 4-nitroanilide hydrochloride provides rapid and accurate results. These measurements are important for diagnosing liver function and other metabolic conditions.
Biochemical Research: In biochemical labs, Nα-Acetyl-L-arginine 4-nitroanilide hydrochloride is utilized to study enzyme kinetics and mechanisms. By using this substrate in kinetic assays, researchers can determine parameters such as Km and Vmax for arginase enzymes. This information is essential for understanding enzyme behavior and for the development of targeted inhibitors.
Pharmaceutical Development: The compound is employed in pharmaceutical research to screen for potential arginase inhibitors that could be used in therapeutic applications. By inhibiting arginase, these compounds may help treat conditions like cardiovascular diseases and certain cancers. Serving as an assay substrate, Nα-Acetyl-L-arginine 4-nitroanilide hydrochloride enables high-throughput screening of drug candidates.
