Nα-Boc-Nε-Z-L-lysine 4-nitrophenyl ester
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Nα-Boc-Nε-Z-L-lysine 4-nitrophenyl ester

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Category
BOC-Amino Acids
Catalog number
BAT-004297
CAS number
2389-46-0
Molecular Formula
C25H31N3O8
Molecular Weight
501.34
Nα-Boc-Nε-Z-L-lysine 4-nitrophenyl ester
IUPAC Name
(4-nitrophenyl) (2S)-2-[(2-methylpropan-2-yl)oxycarbonylamino]-6-(phenylmethoxycarbonylamino)hexanoate
Synonyms
Boc-L-Lys(Z)-ONp
Appearance
Light yellow powder
Purity
≥ 97% (HPLC)
Density
1.234±0.06 g/cm3(Predicted)
Melting Point
79-87 °C
Boiling Point
676.9±55.0 °C(Predicted)
Storage
Store at 2-8 °C
InChI
InChI=1S/C25H31N3O8/c1-25(2,3)36-24(31)27-21(22(29)35-20-14-12-19(13-15-20)28(32)33)11-7-8-16-26-23(30)34-17-18-9-5-4-6-10-18/h4-6,9-10,12-15,21H,7-8,11,16-17H2,1-3H3,(H,26,30)(H,27,31)/t21-/m0/s1
InChI Key
FZHFJIODNQYXRK-NRFANRHFSA-N
Canonical SMILES
CC(C)(C)OC(=O)NC(CCCCNC(=O)OCC1=CC=CC=C1)C(=O)OC2=CC=C(C=C2)[N+](=O)[O-]
2. β-Glucuronidase-coupled assays of glucuronoyl esterases
Lucia Fraňová, Vladimír Puchart, Peter Biely Anal Biochem. 2016 Oct 1;510:114-119. doi: 10.1016/j.ab.2016.07.023. Epub 2016 Jul 22.
Glucuronoyl esterases (GEs) are microbial enzymes with potential to cleave the ester bonds between lignin alcohols and xylan-bound 4-O-methyl-d-glucuronic acid in plant cell walls. This activity renders GEs attractive research targets for biotechnological applications. One of the factors impeding the progress in GE research is the lack of suitable substrates. In this work, we report a facile preparation of methyl esters of chromogenic 4-nitrophenyl and 5-bromo-4-chloro-3-indolyl β-D-glucuronides for qualitative and quantitative GE assay coupled with β-glucuronidase as the auxiliary enzyme. The indolyl derivative affording a blue indigo-type product is suitable for rapid and sensitive assay of GE in commercial preparations as well as for high throughput screening of microorganisms and genomic and metagenomic libraries.
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