2. Isopropanol poisoning
R J Slaughter, R W Mason, D M G Beasley, J A Vale, L J Schep Clin Toxicol (Phila). 2014 Jun;52(5):470-8. doi: 10.3109/15563650.2014.914527. Epub 2014 May 9.
Introduction: Isopropanol is a clear, colorless liquid with a fruity odor and a mild bitter taste. Most commonly found domestically as rubbing alcohol, isopropanol is also found in numerous household and commercial products including cleaners, disinfectants, antifreezes, cosmetics, solvents, inks, and pharmaceuticals. Aim: The aim of this review is to critically review the epidemiology, toxicokinetics, mechanisms of toxicity, clinical features, diagnosis, and management of isopropanol poisoning.
3. Precipitation of DNA with Isopropanol
Michael R Green, Joseph Sambrook Cold Spring Harb Protoc. 2017 Aug 1;2017(8):pdb.prot093385. doi: 10.1101/pdb.prot093385.
DNA is less soluble in solutions containing isopropanol than in solutions containing ethanol. In contrast to precipitation with ethanol, which requires 2-3 volumes of alcohol, precipitation with isopropanol is performed with 0.6-0.7 volume of alcohol. Isopropanol is often the better choice when precipitating DNA from large volumes of solution. Precipitation with isopropanol, described here, is performed at room temperature to lessen the risk that solutes like sucrose or sodium chloride will be coprecipitated with the DNA.
