Pro-Hyp-OH, a collagen peptide composed of proline (Pro) and hydroxyproline (Hyp), has been studied for its potential role in weight loss and anti-aging effects. Research indicates that this peptide can promote the extracellular matrix gene expression in cultured human fibroblasts, which may contribute to its anti-aging properties. Additionally, H-Pro-Hyp-OH has been reported to be an orally active bioactive peptide against UVB-induced skin aging, highlighting its use in cosmetic applications. Furthermore, studies have shown that ingestion of gelatin, which contains peptides like Pro-Hyp, can increase levels of Pro-Hyp in human plasma, suggesting a potential role in improving physical conditions and possibly aiding in weight management. The absorption of these collagen-derived peptides in human plasma implies that they could have systemic effects, including those related to skin health and potentially, body composition. Therefore, H-Pro-Hyp-OH is not only significant in the realm of anti-aging and skin health but also holds promise as a component in weight loss products due to its impact on collagen metabolism and plasma peptide levels.