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This sequence is a N-ethylmaleimide sensitive factor (NSF) peptide that connects to the 11 amino acid cell permeable human immunodeficiency virus (HIV) transactivating regulatory protein (TAT) domain by Gly-Gly-Gly spacer. The peptide contains the NSF domain extending from 222 to 243 amino acids, which is located directly at the amino terminus of the Walker A motif in the NSF D1 domain. ATPase assay shows that TAT-NSF222 inhibits the activity of NSF ATPase.
