2. RNA purification--precipitation methods
Sarah E Walker, Jon Lorsch Methods Enzymol. 2013;530:337-43. doi: 10.1016/B978-0-12-420037-1.00019-1.
When working with RNA, the need often arises to concentrate a sample or purify it from various salts, nucleotides, and proteins. RNA precipitation is an easy and cost-effective method for the concentration of RNA, leaving a pellet that can be resuspended in the buffer of choice.
3. Acetate as a potential feedstock for the production of value-added chemicals: Metabolism and applications
Yeonhee Kim, Suman Lama, Deepti Agrawal, Vinod Kumar, Sunghoon Park Biotechnol Adv. 2021 Jul-Aug;49:107736. doi: 10.1016/j.biotechadv.2021.107736. Epub 2021 Mar 26.
Acetate is regarded as a promising carbon feedstock in biological production owing to its possible derivation from C1 gases such as CO, CO2 and methane. To best use of acetate, comprehensive understanding of acetate metabolisms from genes and enzymes to pathways and regulations is needed. This review aims to provide an overview on the potential of acetate as carbon feedstock for industrial biotechnology. Biochemical, microbial and biotechnological aspects of acetate metabolism are described. Especially, the current state-of-the art in the production of value-added chemicals from acetate is summarized. Challenges and future perspectives are also provided.
