Z-L-pyroglutamic acid 4-nitrophenyl ester
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Z-L-pyroglutamic acid 4-nitrophenyl ester

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Category
CBZ-Amino Acids
Catalog number
BAT-005778
CAS number
40356-52-3
Molecular Formula
C19H16N2O7
Molecular Weight
384.34
Z-L-pyroglutamic acid 4-nitrophenyl ester
IUPAC Name
1-O-benzyl 2-O-(4-nitrophenyl) (2S)-5-oxopyrrolidine-1,2-dicarboxylate
Synonyms
Z-L-Pyr-ONp
Appearance
Pale yellowish powder
Purity
≥ 98% (TLC)
Melting Point
142-144 °C
Storage
Store at 2-8°C
InChI
InChI=1S/C19H16N2O7/c22-17-11-10-16(18(23)28-15-8-6-14(7-9-15)21(25)26)20(17)19(24)27-12-13-4-2-1-3-5-13/h1-9,16H,10-12H2/t16-/m0/s1
InChI Key
ZRQOXQJWPSHSDJ-INIZCTEOSA-N
Canonical SMILES
C1CC(=O)N(C1C(=O)OC2=CC=C(C=C2)[N+](=O)[O-])C(=O)OCC3=CC=CC=C3
2. Pitfalls of assays devoted to evaluation of oxidative stress induced by inorganic nanoparticles
Juliana Tournebize, Anne Sapin-Minet, Grzegorz Bartosz, Pierre Leroy, Ariane Boudier Talanta. 2013 Nov 15;116:753-63. doi: 10.1016/j.talanta.2013.07.077. Epub 2013 Aug 7.
During the last years, there has been a remarkable increase in the use of inorganic nanoparticles (NP) in different applications, including consumer and medical products. Despite these promising applications, the extremely small size of NP allows them to penetrate cells, in which they can interact with intracellular structures causing serious side effects. A number of studies showed that NP cause adverse effects predominantly via induction of an oxidative stress - an imbalance between damaging oxidants and protective antioxidants - resulting in inflammation, immune response, cell damages, genotoxicity, etc … Most of the in vitro methods used for measurement of oxidative stress biomarkers were designed and standardized for conventional organic, inorganic and biochemical compounds. More recently, these methods have been adapted to studies related to various nanomaterials. Thus, this review is an attempt to highlight some current methods employed in and to provide a critical analysis of the major challenges and issues faced in this emerging field.
3. β-Glucuronidase-coupled assays of glucuronoyl esterases
Lucia Fraňová, Vladimír Puchart, Peter Biely Anal Biochem. 2016 Oct 1;510:114-119. doi: 10.1016/j.ab.2016.07.023. Epub 2016 Jul 22.
Glucuronoyl esterases (GEs) are microbial enzymes with potential to cleave the ester bonds between lignin alcohols and xylan-bound 4-O-methyl-d-glucuronic acid in plant cell walls. This activity renders GEs attractive research targets for biotechnological applications. One of the factors impeding the progress in GE research is the lack of suitable substrates. In this work, we report a facile preparation of methyl esters of chromogenic 4-nitrophenyl and 5-bromo-4-chloro-3-indolyl β-D-glucuronides for qualitative and quantitative GE assay coupled with β-glucuronidase as the auxiliary enzyme. The indolyl derivative affording a blue indigo-type product is suitable for rapid and sensitive assay of GE in commercial preparations as well as for high throughput screening of microorganisms and genomic and metagenomic libraries.
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