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BOC Sciences provides technical articles related to amino acids, peptides, and peptide nucleic acids (PNA).
Amino acids，a class of organic compounds that contain amino and carboxyl groups are one of the many biologically active macromolecules that build biological organisms, and are the basic materials for building cells and repairing tissues. With a variety of special functional groups and diverse structures, amino acids have a wide range of applications in pharmaceutical, food, cosmetic and other fields.
Amino acid analysis techniques include paper chromatography and thin layer chromatography, gas chromatography, high performance liquid chromatography, capillary electrophoresis, high performance anion exchange chromatography-integrated pulsed amperometric detection (HPAEC-IPA), etc.ric detection (HPAEC-IPA), etc.
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Amino acids have been widely used in industrialized production, covering multiple industries such as food and health care, medicine, agriculture, and animal husbandry. In industry, amino acid synthesis methods are mainly divided into the following types: enzymatic method, extraction method, chemical synthesis method, fermentation method, etc.
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Bioorthogonal non-canonical amino acid tagging (BONCAT), based on intracellular introducing orthogonal chemoselective groups of small molecules, can realize specifically labeling, qualitative and quantitative analysis of newly translated proteins. etc.
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According to their molecular structure, peptide synthetic reagents can be roughly divided into carbodiimides, phosphonium salts, uronium salts, organophosphorus, and other types of coupling reagents. Among them, carbodiimides, uronium salts, and phosphonium salts are the major three categories. Carbodiimides are first developed and most commonly used while uronium salts and phosphonium salts perform best with wide applications.
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