BOC Sciences has rich experience in synthesis services, and provides professional customized acetylation modification services based on amino acid synthesis, peptide synthesis, chromatography, spectroscopy and mass spectrometry technical services.
Acetylation modification refers to the process in which the acetyl group of the donor (such as acetyl-CoA) is transferred to the terminal amino acid residue (α amino group) of the acceptor protein or the lysine residue (ε amino group) in the chain under the action of an enzyme (or non-enzyme) group. Acetylation modification is closely related to the regulation of protein activity. Acetylation modifications on histones are crucial in the process of epigenetic regulation. Acetylation modification of non-histone proteins affects mRNA stability, protein localization and interaction, and degradation processes, and is related to tumorigenesis, cancer cell proliferation, and immune effects. Acetylation modifications are mainly divided into two categories: acetylation modifications occurring at the N-terminus of amino acids, and acetylation modifications occurring on lysine residues in the amino side chain. N-acetylation is the most common protein modification in eukaryotic protein modification. Lysine acetylation is a reversible process and is an important modification of chromatin proteins in transcription-related processes.
Biological function of acetylation
Although the advancement of proteomics research methods has led to a deeper understanding of acetylation modification, there are still many unknowns about the interaction and regulation between acetylation proteomes that need to be explored urgently. In addition, as an important fine organic chemical intermediate, acetyl amino acid is widely used in medicine, pesticide, chemical industry, food, feed and other fields. We provide professional acetylation modification identification services and amino acid acetylation modification synthesis services to meet the different needs of customers.
Relying on the professional HPLC-MS/MS mass spectrometry platform, we provide efficient and professional acetylation identification services, which can analyze various eukaryotic and prokaryotic samples. Our analysis steps include: (1) Proteolysis in gel or in solution. (2) Enrichment of acetylated peptides. (3) Separation by HPLC followed by MS/MS analysis. (4) Data analysis of acetylation sites. This service can perform qualitative and relative quantitative analysis of acetylation sites on various proteins, determine the site where specific modifications occur, and determine the sequence information of specific modified peptides. The analysis result is reliable, and the operation is simple and convenient.
Acetylation of amino acids refers to the process of transferring acetyl groups to amino acid side chain groups.
Alkaline acylation is the main acetylation technique for amino acids. The specific method is to acetylate the amino acid with an acetylating reagent at a certain temperature under alkaline conditions, and after the acylation reaction is completed, the reaction solution is concentrated and crystallized at low temperature to finally obtain the target N-acetylated amino acid. Commonly used acetylation reagents include acetyl chloride, acetic anhydride, and glacial acetic acid, among which glacial acetic acid is the cheapest and most readily available, and acetyl chloride reacts the fastest. The base used includes aqueous sodium hydroxide solution, weakly basic sodium salt, reactant amine, and the like. Alkaline acylation has a high conversion rate, and the reaction conditions are mild and easy to control.
Acetic acid is used instead of dilute alkali solution as a reaction medium, and amino acid reacts with acetylating reagent to obtain N-acetyl amino acid. Suitable amino acids include methionine, phenylalanine, alanine. The amount of reagents used in the method is small, and the production cost is greatly reduced. Moreover, the product yield is high and the purity is high, which is beneficial to the follow-up process.