Amino acid analysis (AAA) involves identifying amino acid types and quantities. It can be utilized to assess the amino acid makeup of proteins, peptides, and protein drug samples, determine protein levels post-hydrolysis, or characterize proteins through peptide mass fingerprinting or in conjunction with MS/MS sequencing. BOC Sciences employs GC/MS and LC/MS platforms to offer comprehensive amino acid analysis services, enabling both quantitative and qualitative analysis of over 70 amino acids and their derivatives. We always put the quality of its scientific research service on top, prioritizes the needs of our customers, performs our analysis under strict procedures, and provides our customers with professional scientific analysis of the research and development services.
BOC Sciences' amino acid analysis solutions provide you with a way to control the main factors that change your amino acid analysis. From optimized instrumentation and quality control chemistry methods to standardized software engineering, methods, report templates, and qualification tools, you can rely on a comprehensive, detailed, robust, and reliable analytical method to ensure successful analysis of every sample.
Amino acid composition analysis can qualitatively and quantitatively determine the amino acid composition in a sample. We use amino acid automatic analyzer and high-performance liquid chromatography to determine the amino acid composition of various samples. Our one-stop service includes processing and analysis of samples provided by customers, analysis of acquired data and completion of project reports.
We provide N-terminal sequencing services based on mass spectrometry technology and the Edman degradation method, which can achieve accurate interpretation of N-terminal amino acid sequences and ensure the coverage and accuracy of sequencing results. In addition, N-terminal sequencing based on mass spectrometry is complementary to Edman degradation sequencing, which can realize the determination of N-terminal blocked amino acid sequences.
We provide comprehensive C-terminal sequencing services for various samples using mass spectrometry, carboxypeptidase digestion, and chemical reagent methods. Mass spectrometry enables accurate identification of C-terminal residues, and sequencing can typically be completed within 4–5 weeks, supporting protein characterization, structural analysis, and related research needs.
We have a professional amino acid analysis laboratory, which can use a variety of derivatization methods to convert amino acid molecules into substances that absorb ultraviolet and visible light or produce fluorescence. We performed qualitative and quantitative analysis of derivatized amino acid derivatives, solved the difficulties in amino acid analysis, and improved the sensitivity and resolution of amino acid analysis methods.
Without adding ion-pair reagents, we derivatize a large number of amino acids through different separation modes such as reversed-phase chromatography mode, hydrophilic interaction chromatography (HILIC) mode, and ion exchange mode. In addition, we can add volatile ion-pairing reagents to the mobile phase system to perform derivatized direct analysis of amino acids in clinical blood samples.
We use normal-phase or reverse-phase polysaccharide chiral columns to analyze amino acids protected by groups such as Boc or FMOC, and use ligand-exchange chiral columns to analyze unprotected amino acids. Appropriate mass spectrometry methods as well as capillary electrophoresis based on chiral ligand exchange also enable chiral separation and analysis of amino acids.
We can provide high-resolution, high-sensitivity, high-speed, high-throughput UPLC amino acid analysis solutions, and issue UPLC analysis and detection data in a short time. Our UPLC amino acid analysis solution is suitable for the characterization of amino acids in peptides and proteins and the analysis of amino acids in food, feed, pharmaceuticals and cosmetics.
With years of experience in mass spectrometry services, we use a variety of mass spectrometry techniques to identify the type and molecular weight of amino acids, and identify the composition and arrangement information of amino acids. We can detect regular free amino acids as well as amino acids of physiological importance. In addition, we can also customize the detection of special amino acids according to the specific needs of customers.
We offer de novo peptide sequencing services based on high-resolution tandem mass spectrometry, enabling the identification of unknown peptide sequences without the need for a reference database. This service delivers high accuracy, can identify modified residues, and is ideal for antibody sequencing, natural peptide identification, and proteomics research, supporting drug development and functional studies.
BOC Sciences possesses extensive technical expertise and solutions in amino acid quality research and amino acid composition drugs. This includes setting standards for starting materials of active pharmaceutical ingredients, conducting quality research and filing for active pharmaceutical ingredients, developing amino acid preparation prescriptions, and conducting quality research on peptide/protein drugs (such as amino acid composition, extinction coefficient, sequence determination, D-configuration determination, peptide mapping analysis, etc.).
Sample Receipt and Information Confirmation
After receiving the sample, we verify the sample details and analysis requirements to ensure clarity of the analysis objectives and sample type.
Sample Pre-treatment and Preparation
Based on the nature of the sample, appropriate pre-treatment methods (such as hydrolysis, filtration, or extraction) are applied to prepare the sample solution suitable for analysis.
Derivatization and Chromatographic Separation
Amino acids in the sample are derivatized to enhance detection sensitivity and separation efficiency, followed by separation and analysis using HPLC or GC.
Data Acquisition and Processing
Chromatograms and peak area data are automatically collected by the instrument, and amino acids are qualitatively identified and quantitatively determined using standard references.
Data Analysis and Report Preparation
Professional analysts conduct in-depth analysis of the results and prepare a detailed test report, including amino acid content, composition, and relevant evaluations.
Report Review and Customer Feedback
The quality control team rigorously reviews the report for accuracy before delivery; after sending, customer feedback is actively collected to continuously improve service quality.
In the pharmaceutical field, amino acid analysis is a critical part of production and quality control. It is used to detect amino acid components and impurities in drugs, ensuring drug purity and stability. This is especially important in biopharmaceuticals and peptide drug development to guarantee efficacy and safety.
Amino acid analysis is an important tool for studying cellular metabolism, protein synthesis, and disease mechanisms. It is used to measure amino acid levels in body fluids and tissue samples, assisting in disease diagnosis and biomarker discovery, thus advancing precision medicine and drug development.
In protein design and synthesis, amino acid analysis is used to confirm the accuracy and completeness of synthesized proteins. It helps optimize protein structure and function, promotes the development of novel enzymes and biomaterials, and facilitates advances in life sciences technology.
Amino acid analysis is applied in microbial metabolism studies to reveal mechanisms of amino acid synthesis and utilization in microorganisms. It aids in screening industrial fermentation strains, optimizing production processes, and improving yield and quality of bioproducts.
Amino acid analysis is used in the food industry to assess protein content and amino acid composition, ensuring nutritional value and quality safety. It helps formulate recipes, detect food adulteration, and optimize fermentation processes, enhancing product taste and nutritional performance.
In agriculture, amino acid analysis evaluates feed quality and crop protein content, guiding animal nutrition balancing and fertilizer improvement. By monitoring amino acid changes in plants and soil, it helps optimize crop growth environments and improve yield and quality.
Amino acid analysis is a technique that quantitatively determines the composition and content of amino acids in a sample through chemical or instrumental methods. It is widely applied in protein identification, nutritional evaluation, and quality control of bioproducts.
Amino acid composition, mass spectrometry, and amino acid sequence determination are of great significance to confirm the structure of peptides, and amino acid composition analysis is an investigation item that must be added to the quality research of peptide drugs and their raw materials. The following are some techniques commonly used for amino acid analysis:
Typically, samples require hydrolysis (such as acid hydrolysis or enzymatic hydrolysis) to release free amino acids, followed by derivatization to enhance detection sensitivity, and then separation and detection by instruments.
Derivatization improves the volatility, stability, and detection sensitivity of amino acids, facilitating chromatographic separation and quantitative analysis by detection instruments such as fluorescence detectors or mass spectrometers.
Accuracy is ensured by using standard calibration, selecting appropriate internal standards, strictly controlling sample preparation conditions, maintaining and calibrating instruments, and performing replicate experiments to verify data consistency.
Most standard amino acids can be detected; however, certain amino acids such as tryptophan and cysteine are easily degraded under conventional acid hydrolysis conditions and require special treatment methods.
