Amino Acid Analysis
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Amino Acid Analysis

BOC Sciences always places the quality of its scientific research service on top, prioritizes the needs of our customers, performs our analysis under strict procedures, and provides our customers with professional scientific analysis of the research and development services.

Amino-Acid-Analysis

Introduction

Amino acid is the basic unit of protein. Its wide range of use can be applied to areas such as food, medicine, feed additives, cosmetics, industry and agriculture. Development in bioengineering technologies has helped amino acid to gradually become one of the major research areas in the 21st century. Demand for amino acids is increasing. and new reforming processes of the amino acids are emerging. In recent years, more attentions are given to the innovation and improvement of amino acid analysis methods. The speed, sensitivity, and instrument automation levels of amino acid analysis have advanced. At present, instruments used to determine amino acids mostly consist of ion exchange chromatography (IEC), high performance liquid chromatography (HPLC), or gas chromatography (GC). The detectors used in these instruments include ultraviolet-visible absorption, fluorescence, chemiluminescence, etc.

Application

Technologies used to analyze amino acid are important for life science, food science, clinical medicine, geological investigation, paleontology and other fields. Amino acid analysis has a wide range of applications. For example, to cultivate excellent varieties of crops, the content of protein and amino acids in the seed must be considered. The content of methionine in the research of feed formula must be determined. Diseases can be diagnosed based on the change of some amino acid content in physiological fluids. BOC Sciences uses UPLC® amino acid analysis solution to meet specific needs, such as: characterization of amino acids in proteins, monitoring of cell culture media, and analysis of nutritional components in food and feed. This type of end-to-end system can successfully provide accurate and reliable data information.

Our Services

BOC Sciences's amino acid analysis solutions provide you with a way to control the main factors that change your amino acid analysis. From optimized instrumentation and quality control chemistry methods to standardized software engineering, methods, report templates, and qualification tools, you can rely on a comprehensive, detailed, robust, and reliable analytical method to ensure successful analysis of every sample.

Our services include but not limited to the following):

Amino acid composition analysis can qualitatively and quantitatively determine the amino acid composition in a sample. We use amino acid automatic analyzer and high performance liquid chromatography to determine the amino acid composition of various samples. Our one-stop service includes processing and analysis of samples provided by customers, analysis of acquired data and completion of project reports.

We provide N-terminal sequencing services based on mass spectrometry technology and Edman degradation method, which can achieve accurate interpretation of N-terminal amino acid sequences and ensure the coverage and accuracy of sequencing results. The N-terminal sequencing based on the Edman degradation method can realize the determination of 20-40 amino acids at a time. N-terminal sequencing based on mass spectrometry is complementary to Edman degradation sequencing, which can realize the determination of N-terminal blocked amino acid sequence.

We can provide comprehensive C-terminal sequencing services for various samples. The methods of C-terminal sequencing include mass spectrometry, carboxypeptidase method and chemical reagent method. We can complete amino acid C-terminal sequencing within 4-5 weeks using mass spectrometry.

We have a professional amino acid analysis laboratory, which can use a variety of derivatization methods to convert amino acid molecules into substances that absorb ultraviolet and visible light or produce fluorescence. We performed qualitative and quantitative analysis of derivatized amino acid derivatives, solved the difficulties in amino acid analysis, and improved the sensitivity and resolution of amino acid analysis methods.

Without adding ion-pair reagents, we derivatize a large number of amino acids through different separation modes such as reversed-phase chromatography mode, hydrophilic interaction chromatography (HILIC) mode, and ion exchange mode. In addition, we can add volatile ion-pairing reagents to the mobile phase system to perform derivatized direct analysis of amino acids in clinical blood samples.

We employ a variety of analytical methods to provide high-quality solutions for the chiral analysis of protected and unprotected amino acids. We use normal-phase or reverse-phase polysaccharide chiral columns to analyze amino acids protected by groups such as Boc or FMOC, and use ligand-exchange chiral columns to analyze unprotected amino acids. Appropriate mass spectrometry methods as well as capillary electrophoresis based on chiral ligand exchange also enable chiral separation and analysis of amino acids.

We can provide high-resolution, high-sensitivity, high-speed, high-throughput UPLC amino acid analysis solutions, and issue UPLC analysis and detection data in a short time. Our UPLC amino acid analysis solution is suitable for the characterization of amino acids in peptides and proteins and the analysis of amino acids in food, feed, pharmaceuticals and cosmetics.

With years of experience in mass spectrometry services, we use a variety of mass spectrometry techniques to identify the type and molecular weight of amino acids, and identify the composition and arrangement information of amino acids. We can detect regular free amino acids as well as amino acids of physiological importance. In addition, we can also customize the detection of special amino acids according to the specific needs of customers.

Our advantages

  • High sensitivity down to low microgram scale
  • Amino acid testing of all 20 natural amino acids
  • Comprehensive report delivered within 5-7 days
  • Analysis of non-standard amino acids
  • Stable and reliable quality
  • Competitive cost

Reference:

  1. Piestansky J, Galba J, Olesova D, Kovacech B, Kovac A. Determination of immunogenic proteins in biopharmaceuticals by UHPLC-MS amino acid analysis. BMC Chem. 2019;13(1):64. Published 2019 May 10. doi:10.1186/s13065-019-0581-z
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