As a professional peptide products and services company we deliver high-quality biotinylation services to support global research and biopharmaceutical industries. Our company combines advanced synthesis technologies with rigorous quality control and a professional R&D team to accurately biotinylate peptides resulting in products with high activity, stability and specificity. We provide solutions for multiple applications such as drug development as well as diagnostic reagent development and biotechnology research. Our one-stop customized service covers peptide design through synthesis and biotinylation and includes post-modification quality testing and analysis.
N-terminal Modification: Biotin can be directly labeled at the N-terminal of the peptide, such as Biotin (N-terminal, Y/N Ahx), in various forms.
C-terminal Modification: Biotin can be attached to the C-terminal of the peptide to meet specific application requirements for modification sites.
Lysine Residue Modification: When the peptide sequence contains lysine, biotin can be modified onto the lysine side chain, such as Biotin (with Lys in the sequence). If lysine is absent in the sequence, biotin can be connected to other suitable locations on the peptide via specific chemical reactions, such as Biotin (without Lys in the sequence).
Standard Biotinylation: This is the most common and cost-effective biotinylation method, suitable for most routine biotin labeling needs.
Photoaffinity Biotinylation: This method combines biotin with specific photo-reactive groups. Under light exposure, it enables covalent binding with target biomolecules, facilitating more stable and precise molecular recognition and capture under specific conditions.
Fluorescent Group-Conjugated Biotinylation: This involves labeling peptides with both biotin and a fluorescent group, such as FITC. It provides the high affinity and specificity of biotin while allowing detection and localization via fluorescence signals, suitable for more complex and diverse experimental needs.
Custom Modification Sites: Based on the customer's specific experimental needs and peptide sequence characteristics, we design and synthesize peptides with biotin modified at specific positions, such as at a certain amino acid side chain within the peptide.
Custom Modification Levels: According to customer requirements, we provide peptides with varying degrees of biotinylation, such as single or multiple biotin modifications, to meet different experimental goals and application scenarios.
Custom Special Requirements: For special experimental needs, such as requiring additional modifications or reactions on top of the biotinylation, we offer personalized services based on the customer’s detailed instructions.
Peptide Synthesis and Purification: We provide high-quality peptide synthesis services, using advanced synthesis technologies and equipment to ensure peptide purity and quality. We strictly control biotinylation efficiency and accuracy during the synthesis process, delivering high-purity, high-activity modified peptides.
Quality Analysis and Testing: Equipped with comprehensive quality testing facilities and technicians, we conduct thorough quality analysis on the modified peptides, including molecular weight determination, purity testing, and biotinylation efficiency. We provide detailed quality inspection reports to ensure the delivered products meet customer quality requirements.
Technical Support and Consultation: Our professional technical team offers comprehensive support and consultation services, covering peptide design, modification selection, and subsequent application. We assist customers in addressing any issues encountered during the use of biotinylation services and help them conduct their research more effectively.
Communication and Evaluation of Needs
The client provides details on the peptide biotinylation, including sequence, length, purity, modification sites, delivery time, and special requests. The service provider reviews these requirements, evaluates feasibility, and provides professional advice along with an initial quote.
Contract Signing and Payment
Once both parties agree on the service content, delivery standards, price, delivery time, and other details, a contract is signed. The client pays a certain percentage of the prepayment.
Peptide Synthesis and Modification
The service provider synthesizes the peptide using methods like solid-phase or liquid-phase synthesis. Biotinylation is performed either during or after synthesis, applying the right method to add biotin to specific parts of the peptide.
Quality Testing
After modification, the peptide undergoes tests for purity (HPLC), molecular weight (mass spectrometry), and biological activity (e.g., binding tests with streptavidin). These tests ensure the peptide meets the client's requirements and quality standards.
Delivery and After-Sales Service
The biotinylated peptide is packaged and delivered as per the schedule, along with a quality report and technical documentation. After-sales support is provided to address any issues and offer technical assistance.
Professional Technical TeamOur team of experts in peptide synthesis and modification is highly skilled and experienced. We specialize in peptide biotinylation techniques to ensure efficient and accurate modifications, delivering reliable, high-quality products.
Efficient Synthesis and ModificationWe use advanced equipment to optimize the synthesis and modification process, improving efficiency and yield.
High Success RateBy refining modification techniques, we achieve a high success rate in biotinylation, ensuring product consistency and reducing client costs.
Strict Quality ControlWe use advanced testing tools like HPLC, mass spectrometry, and thin-layer chromatography to rigorously test peptide purity, molecular weight, and biological activity, ensuring products meet client and industry standards. Detailed inspection reports are provided for transparency.
Diverse Service CustomizationWe offer customized biotinylation services based on client needs, including modifications at various positions, peptide length, sequence, and additional modifications like amidation or phosphorylation.
Comprehensive After-Sales ServiceOur after-sales service includes full technical support and prompt solutions to any issues clients may face, ensuring successful experiments and research projects.
Strong ConfidentialityWe protect client intellectual property with strict confidentiality measures, safeguarding peptide sequences, order details, and other sensitive information to ensure clients' business secrets remain secure.
In medical testing, biotinylated peptides can be used to label antibodies, antigens, or nucleic acid probes, enhancing detection signals and improving sensitivity and accuracy. For example, in enzyme-linked immunosorbent assay (ELISA), biotinylated peptides bind to antibodies, and streptavidin-enzyme conjugates are added. The enzyme catalyzes a color change in the substrate, enabling precise detection of specific antigens or antibodies in biological samples. This method is used for early diagnosis, disease monitoring, and prognosis evaluation.
Biotinylated peptides can be used to study interactions between peptide drugs and biological targets, such as cell membrane receptors and enzymes. By observing the binding of biotinylated peptides to these targets, researchers can gain insights into the drug's mechanism of action, affinity, and specificity, aiding drug design and optimization. Additionally, biotinylation can be employed to develop novel peptide drug delivery systems that improve drug stability, targeting ability, therapeutic efficacy, and reduce side effects.
In gene therapy, biotinylated peptides can be used to construct gene carriers that efficiently deliver therapeutic genes to target cells. The biotinylated peptides bind to cell surface receptors, facilitating the internalization of gene carriers and enhancing gene transfection efficiency. This approach offers new strategies and methods for the treatment of genetic diseases.
Biotinylated peptides can be used to study protein-protein and protein-peptide interactions. By incubating biotinylated peptides with target proteins and utilizing the high-affinity interaction between biotin and streptavidin, proteins binding to the peptides can be isolated and enriched. Techniques such as mass spectrometry can then be used for identification and analysis, revealing protein interaction networks and signaling pathways.
Biotinylated peptides can aid in studying the relationship between protein structure and function. For example, by introducing biotin modifications at various regions of a protein and using techniques such as nuclear magnetic resonance (NMR) and X-ray crystallography, researchers can investigate the protein's three-dimensional structure, folding patterns, and conformational changes related to its biological function, providing a structural basis for understanding its mechanism of action.
In cell biology, biotinylated peptides can be used for labeling and localization studies of cell surface proteins. By binding biotinylated peptides to specific cell surface proteins and detecting them with fluorescently labeled streptavidin, researchers can observe the distribution and expression of proteins at the cellular level. This helps in studying cellular physiological processes and signal transduction pathways.
Biotinylated peptides have broad applications in molecular biology experiments, such as studying nucleic acid-protein interactions, identifying transcription factors, and analyzing gene expression regulation. By binding biotinylated peptides to nucleic acid probes or nucleotides, researchers can isolate and analyze proteins interacting with them, thus exploring the molecular mechanisms of gene expression regulation.
In food testing, biotinylated peptides can be used to detect harmful substances in food, such as contaminants, allergens, and microorganisms. By binding biotinylated peptides to specific antibodies or nucleic acid probes, high-sensitivity detection of target substances in food is achieved, ensuring food safety.
In agriculture, biotinylated peptides can be used for detecting and controlling plant diseases. For instance, biosensors based on biotinylated peptides can be developed to detect plant pathogen infections, enabling early disease detection and timely interventions. This approach reduces pesticide use and improves crop yield and quality.
Biotinylation of peptides involves covalently attaching biotin molecules to specific sites on the peptide through chemical reactions. Common modification sites include the N-terminus, C-terminus, or the amino group on lysine side chains. There are various modification methods, such as isocyanate reaction and NHS ester reaction.
Yes, we offer customization services. You can provide specific peptide sequences and modification requirements based on your experimental needs, and we will synthesize and modify the peptides according to your specifications.
We use advanced synthesis and purification technologies and follow strict quality control processes to ensure the quality of our products. Quality control measures include purity testing by high-performance liquid chromatography (HPLC) and molecular weight identification by mass spectrometry (MS) to ensure the peptides meet customer requirements.
