BOC Sciences is a company dedicated to amino acid research, providing advanced capillary electrophoresis resolution services. We use advanced techniques to ensure accurate and reliable resolution results. In addition to resolution services, we also provide professional related consulting services to help customers solve technical and scientific problems.
Chirality is an important feature of biological systems. A large number of compounds are racemic enantiomeric mixtures. Enantiomers do not differ significantly in physical properties, but exhibit different effects in physiological and chemical properties. Therefore, the recognition and separation of chiral compounds plays an extremely important role in chemical, clinical, pharmaceutical and other industries. As an important biologically active substance, amino acid is an important unit of biological protein. Most amino acids are chiral, and there are two enantiomers, D and L. L-type amino acids can be absorbed and converted into protein by the human body, while D-type amino acids cannot be absorbed and utilized by the human body. The single enantiomers of chiral amino acids have different physiological activities. Therefore, the separation of amino acids and their derivatives can further elucidate the chemical nature of life processes, and has good application prospects in drug research and biosensors.
Applications of capillary electrophoresis
Capillary electrophoresis is a powerful chiral separation technique that uses the difference in the migration speed of ions under the action of an electric field to separate and analyze components. Because capillary electrophoresis has the advantages of simple operation, less sample consumption, high separation efficiency, less pollution, and low cost, it is widely used in the fields of chiral drugs and chiral biomacromolecules. Capillary electrophoresis has unique advantages in the field of chiral resolution of amino acids. We provide reliable and fast capillary electrophoresis resolution services, which help to obtain highly optically active, high-purity amino acid enantiomers to meet relevant requirements.
We use fluorescent probes to label amino acids and their derivatives, investigate and optimize the resolution conditions (such as pH, temperature, voltage, buffer solution, selection of chiral additives), determine the best separation mode, and realize the chiral resolution of amino acids and their derivatives.
The amino acids we can obtain include but are not limited to chiral amino acids (such as D/L-alanine, aspartic acid, glutamic acid, histidine, leucine, serine, valine) and achiral amino acids.
CZE is the simplest separation mode of capillary electrophoresis. We directly add chiral selectors (such as cyclodextrins, crown ethers, macrocyclic antibiotics, proteins, metal complexes, etc.) into the buffer solution to achieve the resolution of amino acids.
CEC is an effective method for separating chiral amino acids. The chromatographic stationary phase is introduced into the capillary column, which combines the advantages of CE and HPLC.
We add ionic surfactants (such as sodium lauryl sulfate) to the buffer solution to achieve the resolution of amino acids.