BOC Sciences develops a competitive, reasonably priced, and complete service system according to the conditions of different customers, and provides high-quality solutions for the chiral analysis of amino acids. We adhere to customer-centric, advanced concepts, and the services and related products we provide are widely recognized.
Chirality is one of the most important properties of nature. Chiral molecules have the same molecular weight and similar physical and chemical properties, and their chemical structures are mirror images without complete overlap. Life activities are inseparable from chirality. The basic units of biological macromolecules such as amino acids are chiral molecules. Most amino acids have two enantiomers, D and L. L-amino acid is an important nutrient for the human body and is widely used in the fields of food, cosmetics and medicine. The concentration change of D-amino acid is closely related to some important physiological diseases. The chirality of amino acids is a key scientific issue closely related to chemistry, medicine, pharmacy, biology and other research fields. It is of great significance to establish an efficient chiral analysis scheme for amino acids.
The chiral analysis of amino acids has important research significance in life sciences. As an advanced provider of amino acid chiral analysis services, we use a variety of analytical methods to provide comprehensive analysis and detection solutions for protected and unprotected amino acids.
High performance liquid chromatography has the characteristics of rapidity, good separation effect, high detection sensitivity and automatic detection. Due to its unique advantages, HPLC is the most commonly used technique in the field of chiral analysis. According to the structure and properties of the tested amino acids, we choose appropriate chiral chromatographic methods to effectively resolve them. Through existing analytical methods, we use normal-phase or reverse-phase polysaccharide chiral columns to efficiently separate and analyze amino acids protected by groups such as Boc or FMOC, saving time and cost for customers. In addition, we also use ligand-exchange chiral columns to separate and analyze free amino acids (such as DL-alanine, DL-aspartic acid, DL-valine, DL-proline, etc.).
Amino acid chiral analysis based on mass spectrometry is a basic analytical method for peptides and proteins, and is also used for disease diagnosis. Mass spectrometry is fast, accurate, sensitive, and does not require sample controls, and can simultaneously monitor the possibility of the presence of other allosteric amino acids. We use appropriate mass spectrometry methods, apply a wide range of chromatographic columns, derivatization reagents and advanced instrument technology to separate, sensitively analyze and accurately quantify amino acids. Our mass spectrometry options include GC-MS, LC-MS and CE-MS.
Capillary electrophoresis based on chiral ligand exchange has the advantages of simple sample pretreatment, less reagent consumption, short separation time, and high resolution, and is widely used in the chiral analysis of amino acid enantiomers. We utilize chiral selectors for the separation and quantitation of several amino acids such as phenylalanine, tryptophan, tyrosine. Our broad selection of chiral selectors includes cyclodextrins, macrocyclic antibiotics, crown ethers and metal complexes, among others.