The chromatographic separation technology of BOC Sciences can easily obtain mg-kg level optically pure chiral amino acid enantiomers for customers with the lowest cost, the most exquisite technology and the fastest speed. Our instruments and detection systems include preparative and analytical high-performance liquid chromatography, reversed-phase liquid chromatography (RP-LC), ultra-performance convergence chromatography (UPC2), gas chromatography, mass spectrometry, charged aerosol detection (CAD), Photodiode array (PDA) UV, NMR spectroscopy, etc.
The separation of amino acid enantiomers is the basis of life sciences, and is of great significance in the research of proteins and peptides, asymmetric synthesis in organic chemistry, and in the fields of medicine, food, and hygiene. Optically pure amino acids are widely used as chiral drug precursors, health products, food, cosmetics, etc.
Chromatography has the advantages of high efficiency, good selectivity, high sensitivity, simple operation, and low cost, and has become the main tool for chiral separation and analysis. Use chiral substances, such as starch and some artificially synthesized chiral macromolecules, as adsorbents for column chromatography. When a racemic amino acid is passed through a chromatography column, two diastereomeric adsorbates can be produced. According to the degree of adsorption of the adsorbate, the separation of amino acids can be carried out. With chiral technology and amino acid synthesis as our core business, we provide efficient amino acid resolution services.
The most commonly used methods for chromatographic resolution of amino acids are high performance liquid chromatography and high performance capillary electrophoresis.
Mixed DL-amino acid chiral ligand exchange chromatography resolution (silicone bond combination)
There are mainly three types of splits:
(1) Use chiral reagents to derivatize the resolved amino acids to generate diastereoisomers, which can be resolved by traditional achiral HPLC.
(2) A chiral complex modifier is added to the mobile phase, and the HPLC with an achiral stationary phase is used for resolution. The achiral stationary phases we use mainly include ion exchange resins, silica gel, reversed phase silica gel, etc. The metal ions of the chiral complex modifier are mainly copper ions and nickel ions. The ligands of chiral complex modifiers are usually L-amino acids and their derivatives.
(3) Use HPLC with chiral stationary phase for the resolution of amino acids. The chiral stationary phases we use mainly include polysaccharide stationary phases, crown ether bonded stationary phases, and ligand exchange chiral stationary phases. We can use chiral amino acids as immobilized ligands and polymers as carriers to synthesize a variety of ligand-exchange chiral stationary phases to achieve rapid resolution of DL-amino acids.
HPCE is characterized by high separation efficiency, high sensitivity, wide application range, short analysis time, small sample volume required, and multiple separation modes. The amino acid resolution modes we usually use are capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC).
GC analysis of amino acids is inexpensive and easily coupled with mass spectrometry. After derivatization of chiral amino acids, we can use GC to resolve them to obtain different optically pure amino acids.