BOC Sciences has established a complete set of enzymatic resolution processes for amino acids through stereoselective regulation of enzymes and optimization of catalytic processes, and finally obtained a single amino acid enantiomer with high yield and high selectivity.
Amino acids are the basis of protein and also a very important nutrient. Amino acids play very important roles in many fields. Amino acids have a D-enantiomer and an L-enantiomer. The two enantiomers act very differently. Under the physiological action of the human body, the L-enantiomer can be directly utilized by the human body. However, the D-enantiomer cannot be directly used by the human body. It needs to go through a series of transformations in the human body to become an L-type amino acid before it can be used by the human body. So chiral resolution of amino acids is a very necessary work. The enzyme constitutes an asymmetric environment by its active center, which is conducive to the recognition of spin bodies. As natural chiral catalysts, enzymes have potential applications in the synthesis of optically active drugs.
Enzymatic resolution has the advantages of high resolution efficiency, high stereoselectivity, mild reaction conditions, less environmental pollution, low cost, and easy industrialization, and is an effective means for preparing chiral amino acids. This method uses enzymes to catalyze the transfer of specific optical isomers (mostly hydrolysis reactions) to generate another compound with very different physical properties, and then separates it from its enantiomer. We have obtained stable enzymes through strain improvement, optimization of fermentation conditions, immobilization exploration, and research on separation processes, and on this basis, we have realized the production and preparation of optically pure amino acids at low cost.
The two enantiomers of D-amino acid and L-amino acid in racemic amino acids, one of which is converted into other substances under the action of enzymes, while the other isomer remains unchanged. Most amino acids can be resolved enzymatically to obtain optically pure enantiomers.
Enzymatic resolution has the following two methods:
(1) We first derivatize racemic amino acids into their derivatives, such as esterified amino acids, N-acylated amino acids. Then use lipase, esterase, aminoacylase or hydantoinase to selectively hydrolyze the derivative. Finally, the optically pure amino acid obtained after hydrolysis is separated from the reactants by conventional physical and chemical methods.
Resolution of amino acids by aminoacylase
(2) We first derivatize the D-isomer and L-isomer of amino acids, and then add enzymes and other chemical reagents. Under the action of the enzyme, one of the isomer derivatives reacts with the chemical reagent, while the other isomer derivative does not react with the chemical reagent, so as to achieve the purpose of resolution.
Relying on modern biotechnology, chemical synthesis technology and separation and purification technology, BOC Sciences has constructed an enzyme extraction system, using enzymes to split racemic amino acids, and using various methods (chromatography, membrane separation, crystallization, etc.) to separate and purify amino acids.