We offer high-quality stable isotope-labeled peptide synthesis services, tailored to meet the needs of fields such as scientific research, quantitative analysis, proteomics, and metabolomics. By selecting appropriate isotope labels (such as nitrogen-15, carbon-13, hydrogen-2, etc.), we ensure precise labeling and high purity of peptides, while also providing comprehensive purification, analysis, quantitative control, and stability testing services. Whether it’s custom synthesis of specific sequences or peptide fragments, or meeting specific experimental requirements, we provide full technical support and consultation to help you achieve your experimental and research goals.
Custom Synthesis: We offer personalized stable isotope-labeled peptide synthesis services based on specific customer requirements, such as designated amino acid sequences, isotope labeling types (e.g., commonly used N15, C13 labels), and purity requirements.
Scale Synthesis: We have the capability to synthesize stable isotope-labeled peptides in quantities ranging from micrograms and milligrams to kilograms, meeting the needs of customers at various stages of scientific research, drug development, and production.
Single Isotope Labeling: We can provide services to synthesize peptides using a single isotope-labeled amino acid, such as labeling a specific amino acid in the peptide with N15 or C13, to meet the customer’s requirements under specific experimental conditions, simplifying experimental design and data analysis.
Multiple Isotope Labeling: We can achieve combinations of various isotope labels, such as labeling different amino acids in a peptide with N15 and C13, or labeling different positions of the same amino acid with multiple isotopes. This type of multi-isotope labeled peptide has significant applications in complex biomedical research and drug development projects, providing richer information and more precise tracking capabilities.
High-Performance Liquid Chromatography (HPLC) Purification: Using advanced HPLC equipment and expert purification techniques, we purify stable isotope-labeled peptides to a high degree of purity, ensuring that the product meets customer requirements.
Custom Purification Plans: We create personalized purification plans based on customer needs and peptide characteristics. For difficult-to-purify labeled peptides, special purification methods and conditions can be employed to improve efficiency and recovery rate, reduce impurity content, and ensure peptide quality and stability.
Mass Spectrometry (MS) Analysis: Using high-precision mass spectrometers, we determine the molecular weight and confirm the structure of stable isotope-labeled peptides, accurately analyzing the molecular composition and isotope labeling to ensure the synthesized peptide meets the required structure and quality standards.
High-Performance Liquid Chromatography (HPLC) Testing: Through HPLC, we test the purity, content, and impurity levels of peptides, providing detailed HPLC chromatograms and analysis reports to help customers understand the quality status of the product. Additionally, HPLC method development and validation can be provided according to customer requirements for testing specific peptides.
Other Testing Projects: We also offer services such as amino acid composition analysis, circular dichroism (CD) analysis, and nuclear magnetic resonance (NMR) analysis to provide comprehensive characterization and analysis of the structure and properties of stable isotope-labeled peptides, offering customers a more thorough quality assessment.
Needs Communication and Consultation
Customers provide details about their research needs, including peptide sequence, labeling type, purity, and quantity. The service provider offers advice and answers any questions.
Quotation and Contract Signing
The service provider calculates costs and provides a detailed quotation. After discussing terms such as price, delivery time, and quality standards, both parties sign a contract to confirm responsibilities.
Peptide Design and Synthesis Plan Formulation
Our technical team designs the peptide and creates a synthesis plan based on the customer’s requirements, including synthesis methods, labeling strategies, and purification processes.
Synthesis and Labeling
Using advanced technologies like Fmoc solid-phase synthesis, the peptide is synthesized with precise control over conditions to ensure accurate labeling and efficient synthesis.
Purification and Analytical Testing
The peptide is purified to remove impurities, and then tested using methods like HPLC, mass spectrometry, and NMR to verify structure, purity, and molecular weight, ensuring quality standards are met.
Delivery and After-Sales Service
The labeled peptide is delivered in the appropriate format with a detailed quality report and documentation. After-sales support is provided, including technical consultation, product guidance, and quality tracking.
High Accuracy and ReliabilityStable isotope-labeled peptides closely resemble target peptides, making them ideal for improving quantitative analysis accuracy, especially in mass spectrometry. They also help in protein structure and dynamics research, providing better insights into protein properties and functions.
Variety of Labeling OptionsIsotopes like 2H, 13C, and 15N are commonly used for different research needs. For example, 13C and 15N labeling helps study protein structures in NMR, while 15N and other isotopes are used in mass spectrometry for quantitative proteomics.
Wide Range of Amino Acid LabelsStable isotopes can label many amino acids (e.g., Tyr, Thr, Lys, Arg, Glu). Researchers can choose specific labeling sites based on their research needs, supporting detailed studies on protein structure, function, and interactions.
Professional Technical TeamExperienced teams with strong peptide synthesis knowledge ensure smooth and efficient synthesis of stable isotope-labeled peptides. They optimize processes to improve synthesis efficiency and product quality.
Strict Quality ControlAdvanced instruments like HPLC, MS, and NMR are used for thorough quality testing of peptides. Detailed reports are provided to confirm the product’s purity, molecular weight, and overall quality.
One-stop ServiceWe offer a complete service from peptide design to synthesis, labeling, and testing, saving time and reducing the need to work with multiple suppliers. This ensures consistency and reduces potential issues.
Customized ServicesWe provide tailored peptide synthesis services, accommodating specific labeling needs, peptide lengths, and purity requirements to support unique research objectives.
Wide Range of ApplicationsStable isotope-labeled peptides are used in proteomics for quantitative analysis, biomarker discovery, and disease research. In drug development, they help with drug metabolism studies and target discovery, while in biochemical research, they assist in studying protein interactions.
Relative Quantification Analysis: In mass spectrometry-based proteomics research, stable isotope-labeled peptides can be used to compare the relative abundance of proteins in different samples. For example, in cell culture, stable isotope labeling with amino acids in cell culture (SILAC) involves adding stable isotope-labeled amino acids to the cell culture system. After labeling, the peptides are separated, digested, and combined with mass spectrometry for relative quantification of protein expression.
Absolute Quantification Analysis: Using isotope dilution mass spectrometry, stable isotope-labeled peptides serve as internal standards to provide absolute quantification of target proteins or peptides. By measuring the signal intensity ratio between the internal standard peptides and the peptides to be tested, the absolute quantity of the target protein or peptide in the sample can be calculated.
Nuclear Magnetic Resonance (NMR) Research: Stable isotope-labeled peptides can introduce NMR-active nuclei, helping to reduce the complexity of spectra, enabling scientists to obtain new atomic-level interactions and providing more comprehensive information on protein structure, dynamics, and protein-protein interactions.
Dairy Product Testing: Stable isotope-labeled peptides can be used for the quantitative detection of nutritional components in dairy products, such as quantifying casein phosphopeptides, allowing for precise control of dairy product quality.
Allergen Detection: Using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) combined with stable isotope-labeled peptides, allergens in food can be accurately quantified. This includes analyzing allergenic proteins in foods like sesame, seafood, chocolate, and rice.
Drug Metabolism Studies: Stable isotope-labeled peptides serve as tracers to track the metabolism of drugs in the body, providing key data on absorption, distribution, metabolism, and excretion (ADME) for drug development and optimization.
Drug Target Discovery and Validation: Labeling peptides for interaction studies with potential drug targets helps discover and validate new drug targets, accelerating the drug development process.
Biochemical Research: Stable isotope-labeled peptides can be used to study post-translational modifications, conformational changes, stability, and interactions with other biomolecules, helping to deepen understanding of protein function and regulatory mechanisms.
Stable isotope-labeled peptides are peptides that are labeled by introducing stable isotopes (such as nitrogen-15, carbon-13, hydrogen-2, etc.) during the peptide synthesis process. This labeling enhances the traceability of peptides in experiments and is commonly used in mass spectrometry analysis, protein quantification, and metabolic studies.
Stable isotope-labeled peptides are widely used in proteomics, metabolomics, drug discovery, quantitative analysis, biomarker screening, and biomedical research, particularly playing a crucial role in quantitative analysis and biomarker studies.
Our team will help you select the most suitable stable isotope labeling based on your research needs (such as target analytical methods, quantification accuracy requirements, etc.). Common options include nitrogen-15, carbon-13, and hydrogen-2.
The synthesized stable isotope-labeled peptides undergo a purification process to remove impurities. We then use techniques such as high-performance liquid chromatography (HPLC) and mass spectrometry (MS) to analyze the peptides, ensuring that they meet the expected purity and labeling requirements.
Yes, we offer custom services to synthesize stable isotope-labeled peptides with specific sequences according to your research needs, including custom peptide segments and different isotope labeling positions.
Stable isotope-labeled peptides generally have excellent stability. However, we provide stability testing and research services to ensure that the labeled peptides remain stable during storage and use, and we offer recommendations for optimal storage conditions.
