Mass Spectrometric Identification of Amino Acids
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Mass Spectrometric Identification of Amino Acids

With many years of experience in mass spectrometry services, BOC Sciences has a good reputation for the identification of amino acids in various proteins and peptides. The combination of rich experience in mass spectrometry projects and high-level instrument facilities enables us to provide high-quality amino acid mass spectrometry identification services.

Why Mass Spectrometry Identification of Amino Acids?

Amino acids are the basic units of biological functional macromolecular proteins. In addition to being the basic unit of protein, amino acids are also cell signaling molecules involved in the regulation of gene expression. They are crucial to maintaining the homeostasis and balance of the body, and play an important role in regulating the growth and immunity of the body. In addition, amino acids can also be used as raw materials for health products and biological medicines. The amino acid identification method is a method for determining the type and content of amino acids in samples containing free amino acids, peptides or proteins. Amino acid identification is an important content in proteomics research. As a highly selective and sensitive detection tool, mass spectrometry plays an important role in amino acid analysis.

Why Choose Us for Mass Spectrometric Identification of Amino Acids?

The identification of amino acids by mass spectrometry is mainly achieved by deriving molecular weights based on information such as their mass-to-charge ratio signals and ion peak intensities. We utilize a variety of mass spectrometry techniques to identify amino acids. By analyzing the mass spectrum information obtained, we can determine the type and molecular weight of amino acids, and identify the composition and arrangement of amino acids.

Our Mass Spectrometry Technology

  • Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF)

We use MALDI-TOF to directly measure the molecular weight of peptides and proteins to obtain amino acid sequence information.

  • Electron Spray Ionization Mass Spectrometry (ESI-MS)

We use ESI-MS to analyze the multiple charge signals obtained by electrospray to obtain the accurate molecular weight value of the sample as well as the modification site and sequence information of the amino acid.

  • Gas Chromatography-Mass Spectrometry (GC-MS)

We start by derivatizing the amino acids. Derivatization treatments include silylation, alkylation and acylation. Then we extract the derivatized derivatives with organic acid and directly enter the mass spectrometer for detection. GC-MS is mainly used for quantitative analysis of amino acids and identification of amino acid enantiomers.

  • Liquid Chromatography-Mass Spectrometry (LC-MS)

Ion-pair liquid chromatography-tandem mass spectrometry (IP-LC-MS/MS) enables the separation of underivatized amino acids on a reversed-phase C18 column. Hydrophilic Interaction Chromatography-Mass Spectrometry (HILIC-MS) provides high separation efficiency for the detection of amino acids in food.

  • Capillary Electrophoresis-Mass Spectrometry (CE-MS)

CE-MS is a highly selective, sensitive and reliable method for identifying amino acid profiles in complex biological samples with minimal sample processing.

  • Direct-infusion Tandem Mass Spectrometry (Direct-infusion MS-MS)

Direct-infusion MS-MS can be used to detect amino acid changes in blood or urine samples. This method can analyze a large number of samples.

Our Mass Spectrometric Identification Services for Amino Acids

We can analyze and identify peptides or proteins, and obtain the precise molecular weight and amino acid sequence information of the sample.

We currently develop multiple methodologies to identify amino acids. We can detect conventional 20 kinds of free amino acids, as well as about 40 kinds of amino acids with important physiological significance. In addition, we can also customize the detection of special amino acids to meet the specific needs of customers.

Mass Spectrometric Identification for Amino Acids

We can detect a variety of samples, including blood, urine, water, soil, feed, and various animal and plant samples. The liquid sample volume needs to be ≥100ul, and the solid powder sample volume needs to be ≥100mg.

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