Our specialized peptide product services team dedicates itself to providing comprehensive and superior N-terminal modification services for peptides. Our services accommodate your needs at any research phase from exploration to the creation of innovative peptide therapeutics. Through our utilization of advanced technology alongside expert researchers and strict quality control measures we maintain high precision and efficiency throughout each distinct modification process including common acetylation and unique fatty acid alterations plus diverse functional group changes. Our high-quality N-terminal peptide modification solutions are designed to support your advanced research and innovative applications within the peptide field.
Acetylation: The peptide's N-terminal amine becomes acetylated when acetic anhydride or acetyl chloride serves as reagents. The modification increases peptide stability while inhibiting degradation from exopeptidases and lessens immunogenicity besides changing peptide solubility and bioactivity. Peptide drug modifications typically involve this modification method.
Formylation: The N-terminal amine of the peptide undergoes formylation when treated with formic acid or formyl chloride. The modification changes peptide characteristics by affecting charge distribution while also influencing solubility and the way peptides interact with receptors.
Special Functional Group Modifications Biotin Labeling: The chemical connection of biotin occurs at the N-terminal position of the peptide. Peptide capture, separation, detection and tracing applications benefit from biotin's strong binding affinity with streptavidin or avidin. Scientists utilize biotin-labeled peptides to analyze protein-peptide interactions and determine cellular localization.
Fluorescent Labeling: Researchers apply fluorescent dyes like FITC or Rhodamine B to mark the N-terminal end of peptides. Peptides with fluorescent labels show fluorescence at particular wavelengths which assists researchers in tracking and localizing these peptides within cells and tissues. Researchers use this technique to analyze how peptides distribute inside cells and interact with membranes or organelles.
HYNIC and DTPA Labeling: HYNIC and DTPA serve as standard metal chelation compounds. The attachment of these agents to the N-terminal of peptides enables subsequent binding with radioactive metal ions to generate radiolabeled peptide probes that assist in nuclear medicine imaging for tumor diagnosis and localization.
Myristic Acid Modification: The peptide's N-terminal end has a myristic acid molecule attached to it. Peptides modified with this medium-chain fatty acid more effectively penetrate cell membranes thereby improving membrane interaction and cellular uptake. Researchers frequently employ this modification technique when developing peptide therapeutics designed to target intracellular locations.
Palmitic Acid Modification: Modifying the N-terminus of peptides with palmitic acid enhances their hydrophobicity which leads to improved membrane binding and cellular uptake efficiency as well as stability. Research on peptide-membrane interactions and intracellular peptide drug development often employs this modification.
Methylation: The peptide's N-terminus receives a methyl group which changes both its electronic properties and stereoconformation. By changing how peptides interact with other molecules this modification controls their bioactivity and stability.
Succinylation: The N-terminal amine of the peptide undergoes a reaction with succinic anhydride that results in the addition of a succinyl group. The succinylation process modifies a peptide to change its solubility and stability while enhancing bioactivity which improves pharmacokinetic properties.
Benzoylation: Benzoylation of the peptide's N-terminus occurs through the use of benzoyl chloride and other similar reagents. The modification enhances peptide hydrophobicity and stability and provides specific optical properties. The method serves as an important tool for analyzing peptide structures and studying their functions.
| BSA conjugation on N terminal -NH2 | Biotin | 6-FAM | Acetylation | DBCO | Nonanoic acid,C9 |
| KLH conjugation on N terminal -NH2 | Biotin-Ahx | 5-FAM | 5-FAM-Ahx | TCO | Palmitic acid,C16 |
| OVA conjugation on N terminal -NH2 | 6-FAM-Ahx | 2-Abz | 4-Abz | Cy3 | D-Lactic acid |
| Trans-Cinnamic acid | Cy5 | Cy5.5 | Cy7 | DABCYL | Decanoic acid,C10 |
| Trans-Crotonic acid | Dansyl | Dansyl-Ahx | FITC-Ahx | FITC-PEG2 | Stearic acid,C18 |
| 4-Azidobutyric acid | 5-TMR | 6-TMR | Rhodamine B | MCA | (R)-Lipoic acid |
| 4-Azidobutyric acid | 5-TMR | 6-TMR | Rhodamine B | MCA | (R)-Lipoic acid |
| 6-Azidohexanoic acid | 3-Maleimide | 6-Maleimide | SMCC | Acryl | Myristic acid,C14 |
| 2-Azidoacetic acid | Alloc | Benzoyl | CBZ | Fmoc | L-Lactic acid |
| 5-Hexynoicacid | Br-Ac | Cl-Ac | Aminooxy | DOTA | Lauric acid,C12 |
| 2-Mercaptoacetic acid | NOTA | 1-Nap | 2-Nap | Succinylation | Arachidic acid,C20 |
| Propiolic acid | Glutaric acid | Butyric acid,C4 | Hexanoic acid,C6 | Octanoic acid,C8 | Methyltetrazine |
Needs Communication
The customer contacts our sales or technical team with details about the peptide sequence, N-terminal modification type, quantity, purity requirements, and any special needs (e.g., packaging). We review the information, answer questions, and make sure both sides understand the project.
Plan Formulation and Quotation
Our team creates a detailed plan for synthesizing the peptide with the requested modifications, including methods and quality control standards. We then calculate the cost and provide a quote with service fees and delivery timelines.
Contract Signing
Once the customer approves the plan and quote, both parties sign a contract outlining terms like payment, delivery standards, and intellectual property ownership, ensuring smooth collaboration.
Project Initiation
After receiving the customer’s prepayment, we start the project by procuring materials, calibrating instruments, and preparing for peptide synthesis.
Peptide Synthesis and Modification
We synthesize the peptide by adding amino acids one by one in a controlled process, then cleave it from the support and purify it. The N-terminal is then modified with specific chemical groups, ensuring precision and high efficiency.
Quality Testing
The modified peptide undergoes HPLC testing for purity, mass spectrometry to verify molecular weight, and checks for solubility and bioactivity as per customer requirements, ensuring full quality control.
Product Packaging and Delivery
The peptide is packaged according to industry standards and customer needs, ensuring stability during transport. Our logistics team ensures timely and safe delivery.
After-Sales Follow-Up
After delivery, our team contacts the customer to gather feedback and address any concerns. If any issues arise, we coordinate with the technical team to provide solutions and ensure satisfaction.
Professional Team and Rich Experience Our team has years of experience in peptide synthesis and modification. With strong academic backgrounds and practical expertise, we deliver precise, efficient N-terminal modification services for both complex peptides and special requirements, ensuring high-quality and reliable results.
Advanced Technology and Equipment We use advanced peptide synthesizers, modification devices, and high-precision instruments like LC-MS. These ensure precise control of the modification process, improving efficiency and peptide purity, making our products ideal for research or applications.
Strong R&D Capabilities We invest in research to develop new N-terminal modification techniques, offering personalized solutions to improve peptide stability, bioactivity, and solubility. Our innovative methods help clients achieve breakthroughs in their fields.
Strict Quality Control System Our quality control system follows international standards (e.g., GMP). Every step, from raw material procurement to final testing, is carefully controlled, ensuring all modified peptides meet high-quality standards for research and production.
Efficient Customized Services We offer customized services to meet each customer’s specific needs, whether for small-scale research or large-scale production. We ensure fast, high-quality modifications to help clients move their projects forward efficiently and cost-effectively.
High-Quality After-Sales Support We provide comprehensive after-sales support, including technical consultation, quality tracking, and problem-solving. Our professional team ensures prompt responses to any issues, offering free technical assistance or rework services as needed to guarantee customer satisfaction.
Protein Structure and Function: N-terminal modification affects peptide properties like stability, solubility, and interaction with other molecules. Studying these changes helps understand protein folding, stability, and how proteins interact with biomolecules, offering deeper insights for proteomics research.
Biomarker Discovery: N-terminal modifications can be linked to diseases. For example, cancer-related proteins have different N-terminal patterns compared to normal cells, making them potential biomarkers for early diagnosis and disease monitoring.
Improving Stability and Bioavailability: N-terminal modification can protect peptides from degradation, increasing their stability and bioavailability, which leads to better therapeutic effectiveness.
Enhancing Targeting and Efficacy: N-terminal modifications can help peptides target specific cells, reducing side effects and improving their effectiveness, especially in cancer therapy.
Immunoregulation and Vaccine Development: N-terminal modification can reduce unwanted immune reactions and improve the stability and effectiveness of peptides in vaccines, enhancing vaccine efficacy and protection.
Functional Modification and Optimization: N-terminal changes can create proteins with new functions, such as improved enzymatic activity, making them useful in industries like biosensors and enzymes.
Synthetic Biology Applications: N-terminal modifications allow precise control over biomolecules, aiding in the design of systems with specific biological functions for applications in manufacturing and bioenergy.
Regulation of Protein Interactions: N-terminal modifications can affect how proteins bind to other molecules, influencing cell signaling pathways, such as gene expression regulation.
Regulation of Cellular Processes: N-terminal changes can control important cellular processes like proliferation and apoptosis, providing potential targets for cancer treatment.
Development of Biosensors: N-terminal modifications can be used to create biosensors that detect specific molecules or cells, aiding early disease detection.
Molecular Imaging: Attaching imaging markers to the N-terminal of peptides allows for real-time, non-invasive imaging of disease-related targets, helping with diagnosis and monitoring.
N-terminal modification can alter the physicochemical properties of peptides, such as improving stability, increasing solubility, enhancing resistance to enzymatic degradation, prolonging half-life in the body, and regulating biological activity. These modifications make the peptides better suited to specific research or application needs.
The choice of modification type should be determined based on factors such as the peptide's intended application, expected function, the environment it will be in, and interactions with other molecules. For example, if the goal is to enhance peptide stability, acetylation might be chosen; if fluorescent labeling for imaging studies is needed, FITC labeling could be selected.
This depends on the specific modification method and peptide synthesis strategy. Some modifications, like acetylation, can be carried out directly during the solid-phase peptide synthesis process at the N-terminal amino group. However, more complex modifications or labeling processes may require the peptide to be synthesized first, followed by specific chemical or biochemical reactions for N-terminal modification.
After modification, peptides undergo rigorous quality testing, such as high-performance liquid chromatography (HPLC) and mass spectrometry (MS), to ensure their purity and the success of the modification.
