1. A convenient route to N-[2-(Fmoc)aminoethyl]glycine esters and PNA oligomerization using a Bis-N-Boc nucleobase protecting group strategy
Filip Wojciechowski, Robert H E Hudson J Org Chem. 2008 May 16;73(10):3807-16. doi: 10.1021/jo800195j. Epub 2008 Apr 16.
A simple and practical synthesis of the benzyl, allyl, and 4-nitrobenzyl esters of N-[2-(Fmoc)aminoethyl]glycine is described starting from the known N-(2-aminoethyl)glycine. These esters are stored as stable hydrochloride salts and were used in the synthesis of peptide nucleic acid monomers possessing bis-N-Boc-protected nucleobase moieties on the exocyclic amino groups of ethyl cytosin-1-ylacetate, ethyl adenin-9-ylacetate and ethyl (O(6)-benzylguanin-9-yl)acetate. Upon ester hydrolysis, the corresponding nucleobase acetic acids were coupled to N-[2-(Fmoc)aminoethyl]glycine benzyl ester or to N-[2-(Fmoc)aminoethyl]glycine allyl ester in order to retain the O(6) benzyl ether protecting group of guanine. The Fmoc/bis-N-Boc-protected monomers were successfully used in the Fmoc-mediated solid-phase peptide synthesis of mixed sequence 10-mer PNA oligomers and are shown to be a viable alternative to the currently most widely used Fmoc/Bhoc-protected peptide nucleic acid monomers.
2. Ultrasound-assisted catalyst-free phenol-yne reaction for the synthesis of new water-soluble chitosan derivatives and their nanoparticles with enhanced antibacterial properties
Andreii S Kritchenkov, Anton R Egorov, Olga V Volkova, Ilya S Kritchenkov, Aleh V Kurliuk, Tatsiana V Shakola, Victor N Khrustalev Int J Biol Macromol. 2019 Oct 15;139:103-113. doi: 10.1016/j.ijbiomac.2019.07.203. Epub 2019 Jul 30.
This work describes ultrasound-assisted phenol-yne addition of p-hydroxybenzaldehyde and propargylic ester of betaine hydrochloride giving only 2-((3-(4-formylphenoxy)allyl)oxy)-N,N,N-trimethyl-2-oxoethan-1-aminium chloride as a product at 100kHz 300W in water. The ultrasonic assisted phenol-yne addition was enhanced to chitosan chemistry. Phenolic chitosan derivatives were obtained by treatment of chitosan with o-, m- or p-hydroxybenzaldehyde followed by reduction of the formed CN bound by NaBH4. The phenolic chitosan derivatives (phenolic component) were involved in ultrasound-mediated reaction with propargylic ester of betaine hydrochloride (yne component). The reaction led to betaine chitosan derivatives in different degree of substitution as o-, m- and p-isomers. The phenolic and betaine derivatives were tested as antibacterial agents against E. coli in comparison with reference antibiotic Tetracycline. Betaine derivatives showed high antibacterial activity. The most effective polymer was p-isomer of high substituted betaine derivative and its activity was more than 2 times higher than the activity of Tetracycline. The nanoparticles based on this polymer were obtained by ionic gelation method. They had 2Rh 126nm, ξ-potential 20mV and were more effective than the corresponding chitosan derivative.
3. Charge-reversal nanoparticles: novel targeted drug delivery carriers
Xinli Chen, Lisha Liu, Chen Jiang Acta Pharm Sin B. 2016 Jul;6(4):261-7. doi: 10.1016/j.apsb.2016.05.011. Epub 2016 Jun 8.
Spurred by significant progress in materials chemistry and drug delivery, charge-reversal nanocarriers are being developed to deliver anticancer formulations in spatial-, temporal- and dosage-controlled approaches. Charge-reversal nanoparticles can release their drug payload in response to specific stimuli that alter the charge on their surface. They can elude clearance from the circulation and be activated by protonation, enzymatic cleavage, or a molecular conformational change. In this review, we discuss the physiological basis for, and recent advances in the design of charge-reversal nanoparticles that are able to control drug biodistribution in response to specific stimuli, endogenous factors (changes in pH, redox gradients, or enzyme concentration) or exogenous factors (light or thermos-stimulation).
